Power-Pfu Polymerase (Ver. 2.0)High fidelity and productivityPMT (Polymerase Modulator on Temperature) technology Broad range of annealing temperatureMinimize the non-specific amplificationProductsCat.No.ProductSizeP250HelixAmp™ Power-Pfu Polymerase (Ver. 2.0)250 unitsP500HelixAmp™ Power-Pfu Polymerase (Ver. 2.0)500 unitsP2500HelixAmp™ Power-Pfu Polymerase (Ver. 2.0)2,500 unitsP250NHelixAmp™ Power-Pfu Polymerase (Ver. 2.0) (with dNTP Mix)250 unitsP500NHelixAmp™ Power-Pfu Polymerase (Ver. 2.0) (with dNTP Mix)500 unitsP2500NHelixAmp™ Power-Pfu Polymerase (Ver. 2.0) (with dNTP Mix)2,500 unitsDescriptionDataDetailsDocumentsHelixAmp™ Power-Pfu polymerase (ver. 2.0), originated from an extreme thermophile, is ideal for high-fidelity PCR amplification. Due to its intrinsic 3’ to 5’ exonuclease (proof-reading) activity, this DNA polymerase shows high-fidelity and produces blunt ended PCR products. NanoHleix"s PMT(Polymerase modulator on temperature) technology" is applied in the buffer system, which is effective to reduce primer-dimer formation and non-specific amplification during the PCR.HelixAmp™ Power-Pfu polymerase (ver. 2.0)is an engineered enzyme showing much higher productivity and processivity than other proof-reading thermo-stable enzymes (e.g. Pfu DNA polymerase, Vent DNA polymerase etc.) without compromise to the fidelity. For the maximum performance of PCR reactions high-quality dNTP mixture is supplied. The addition of TuneUp™ solution in the reaction mixture is a simple way to optimize the difficult targets of PCR.Figure 1. Various annealing temperature of HelixAmp™ Power-Pfu polymerase. 12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under the various annealing temperature. AT = annealing temperature.    Figure 2. The fidelities of PCR enzymes. The fidelity of HelixAmp™ Power-Pfu polymerase was compared with high fidelity enzymes from other companies and Taq polymerase. Error rate = mutation/bp/duplication. Application High fidelity PCR Site-directed mutagenesis Blunt end PCR cloning   Contents  HelixAmp™ Power-Pfu polymerase (2.5 units/㎕)10X Reaction bufferdNTP mix (each 10 mM)5X TuneUp™ solution   Quality Control Contamination assay for bacterial host DNA Activity assay Sensitivity assay Protein purity analysis   Storage buffer50% Glycerol, 50mM Tris-HCl (pH8.0), 0.1mM EDTA, 1mM DTT, 0.1% Tween 20, 0.1% Nonidet P-40, 1mM PMSF          ManualMSDSBrochure