首页 > NanoHelix > NanoHelix/Taqpolymerase glycerol-free5u/㎕, 50u/㎕/Custom/BGT5

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NanoHelix/Taqpolymerase glycerol-free5u/㎕, 50u/㎕/Custom/BGT5

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BGT5

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NanoHelix

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产品说明

Taq polymerase glycerol-free5u/㎕, 50u/㎕Lyophilization ready for preparation of dried amplification mixtures Robust enzyme for routine PCR reactionsLow bacterial DNA contaminationHigh Yields & High Sensitivity ProductsCat.No.ProductFeatureSizeBGT5Taq polymerase glycerol-free5u/㎕CustomBGT50Taq polymerase glycerol-free50u/㎕CustomDescriptionDataSpecificationDocumentsTaq polymerase glycerol-free is a lyophilization-compatible version of highly purified recombinant standard PCR enzyme. Host DNAs are removed almost entirely from the enzyme to minimize false positive reactions during molecular diagnostic applications. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs. Application Routine PCR and RT-PCR Conventional and Real-Time PCRPCR for molecular diagnosticsManufacture of dried amplification mixtures Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity Temperature optimum: Approximately +75°C Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween-20 Purity: ≥95% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 40U enzyme and human total RNA at 37°C, 1hr) DNA contamination test: [E.coli DNA] less than 1 copy/5U enzyme, [Human DNA] Not detectable Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target) Stability: 24 months at -20°C.

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA