Hot-Taq polymerase5u/㎕, 50u/㎕Chemically modified Taq DNA polymeraseAutomatic hot-start PCR enzymeConvenience of reactions set up at room temperature The highest specificity of PCR amplificationEnzyme for real-time PCR and multiplex PCREnzyme for high [GC] target amplification ProductsCat.No.ProductFeatureSizeBHT5Hot-Taq polymerase5 u/㎕CustomBHT50Hot-Taq polymerase50 u/㎕CustomDescriptionDataSpecificationDocumentsHot-Taq polymerase is a chemically-modified form of purified Taq DNA polymerase and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs.ApplicationHot-start PCRMultiplex PCRReal-time PCRHigh specific and sensitive PCRAmplification of complex DNA and cDNAPCR for molecular diagnosticsFigure 1. The high specificity of Hot-Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Hot-Taq polymerase). The problematic targets of human genomic DNA are successfully amplified with Hot-Taq polymerase. The specific amplifications of COMT gene encoding cathecol-O-methyl transferase (COMT) and APOE gene from human genomic DNA template are shown. Manual hot-start was performed as adding Taq DNA polymerase to reaction mixture at 85℃. M: HelixRuler™ 1 kb(+) DNA ladder.  Figure 2. Hot-Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Hot-Taq polymerse) is highly specific. 100 ng human genomic DNA was used as template. The specificity of Hot-Taq polymerase is shown by the amplification of COMT gene encoding cathecol-O-methyl transferase (COMT) compared with Taq polymerase and an antibody-type hot start Taq polymerase.  Figure 3. Hot-Taq polymerase 5u/㎕, 50u/㎕ (= HelixAmp™ Hot-Taq polymerse) possesses the high activity. The enzyme activity of Hot-Taq polymerase was evaluated by the amplification of COMT, APOE, or SBMA gene from 100 ng human genomic DNA templates with various concentrations of enzyme. The activity comparison was performed with other competitive product of chemically modified hot-start Taq DNA polymerase.  Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity Enzyme activation: 15 min at +95°C Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr)  RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr) Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target) Stability: 24 months at -20°C