首页 > NanoHelix > NanoHelix/Hot-Taqpolymerase glycerol-free5u/㎕, 50u/㎕/Custom/BGHT5

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NanoHelix/Hot-Taqpolymerase glycerol-free5u/㎕, 50u/㎕/Custom/BGHT5

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BGHT5

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产品说明

Hot-Taq polymerase glycerol-free5u/㎕, 50u/㎕Lyophilization-compatible (Glycerol-free)Chemically modified Taq DNA polymeraseAutomatic hot-start PCR enzymeConvenience of reactions set up at room temperature The highest specificity of PCR amplificationEnzyme for real-time PCR and multiplex PCREnzyme for high [GC] target amplificationProductsCat.No.ProductFeatureSizeBGHT5Hot-Taq polymerase glycerol-free5 u/㎕CustomBGHT50Hot-Taq polymerase glycerol-free50 u/㎕CustomDescriptionDataSpecificationDocumentsHot-Taq polymerase glycerol-free is a lyophilization-compatible version of Hot-Taq polymerase, a chemically modified enzyme, and quite suitable for high-specific hot-start PCR, real-time PCR and multiplex PCR. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs. Application Hot-start PCR, Multiplex PCR, Real-time PCRHigh specific and sensitive PCRAmplification of complex DNA and cDNAPCR for molecular diagnostics Manufacture of dried amplification mixtures Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity Enzyme activation: 15 min at +95°C Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Tween-20 DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr) Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target) Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA