首页 > NanoHelix > NanoHelix/Ab+Taqpolymerase5u/㎕, 50u/㎕/Custom/BATA50

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NanoHelix/Ab+Taqpolymerase5u/㎕, 50u/㎕/Custom/BATA50

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BATA50

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NanoHelix

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产品说明

Ab+Taq polymerase5u/㎕, 50u/㎕Anti-Taq antibody complexed Taq DNA polymeraseAutomatic hot-start PCR enzymeFast enzyme activationHigh specific and sensitive PCR enzyme ProductsCat.No.ProductFeatureSizeBATA5Ab+Taq polymerase5u/㎕CustomBATA50Ab+Taq polymerase50u/㎕CustomDescriptionDataSpecificationDocumentsAb+Taq polymerase is an anti-Taq antibody complex form of Taq polymerase and ideal for automatic hot-start PCR. This antibody-mediated hot start polymerase provides highly specific and sensitive PCR amplification. The 10x reaction buffer supplied together contains pH-buffering agent, salts, magnesium, and dNTPs. Application Antibody-mediated Hot-start PCRHigh specific conventional and real-time PCRFast PCR assays with a short enzyme activation time and fast cycling protocolsMultiplex PCRPCR for molecular diagnostics Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activityEnzyme activation: 2 min at +95°CStorage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol.DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr)Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target)Stability: 24 months at -20°C.

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA