Premium-Taq polymeraseMinimize the non-specific amplification by a dual control system;Anti-Taq antibody complexed Taq DNA polymerasePMT technology applied buffer systemHigh specific and sensitive PCR enzymeAutomatic hot-start PCR enzymeFast enzyme activation ProductsCat.No.ProductFeatureSizeBPT5Premium-Taq polymerase5u/㎕CustomBPTCPremium-Taq polymeraseCustomCustomDescriptionDataSpecificationDocumentsPremium-Taq polymerase provides utmost specific amplification by a dual system; antibody-mediated hot start polymerase and PMT (Polymerase Modulator on Temperature)-applied buffer. Anti-Taq antibody inhibits the Taq polymerase activity until it reaches to denaturation temperature and PMT-applied buffer reduces the primer-dimer formation and nonspecific amplification during the PCR cycles. The 5x reaction mix supplied together contains pH-buffering agent, salts, magnesium, dNTPs, and PMT additive.   Application Antibody-mediated Hot-start PCRHigh specific amplification and minimize the primer dimer formationFast PCR assays with a short enzyme activation time and fast cyclingMultiplex PCRPCR for molecular diagnostics  Figure 1. Comparison of Premium-Taq polymerase with other company’s hot start versions of DNA polymerases. 12 different primer sets designed from human genome were used in this PCR. PCR was performed using 10 ng of human genomic DNA under various annealing temperature. A : NanoHelix, B : Company I[Korea], C : Company I[USA], D : Company T[Japan]. Figure 2. Superior performance of Premium-Taq polymerase on difficult targets. Different primer sets (ApoE, MD, Prion, COMT) designed from the indicated region of human genome were used in this PCR. PCRs were performed using 10 ng of human genomic DNA. All of the DNA polymerases are hot-start versions of each brands. A: Company T[Japan], B: Company I[Korea], C: Company B[Korea], D: Company I[USA], E: Company S[Korea], F: Premium-Taq [NanoHelix]. Enzyme activities: Highly processive 5"-3" DNA polymerase; double-strand specific 5"-3" exonuclease; no 3"-5" exonuclease activity Enzyme activation: 2 min at +95°C Storage buffer: 20 mM Tris-HCl (pH 9.0), 1 mM DTT, 0.1 mM EDTA, 100 mM KCl, 0.5% (v/v) Nonidet P40, 0.5% (v/v) Tween-20 and 50% (v/v) glycerol DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr)  RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr) Activity test in PCR and qPCR: Correspond to reference (Human genomic DNA target) Stability: 24 months at -20°C