Thermo Reverse Transcriptase200 u/㎕ , 1,000 u/㎕Modified MMLV RTase, RNaseH negative Active at 42~55℃. Optimum at 50℃High processivity (Up to 12 kb or more) and sensitivity Superior performance in RT-PCRProductsCat.No.ProductFeatureSizeBRT200Thermo Reverse Transcriptase200 u/㎕CustomBRT1000Thermo Reverse Transcriptase1,000 u/㎕CustomDescriptionDataSpecificationDocumentsThermo Reverse Transcriptase, a thermostable and RNase H-negative variant of M-MLV Reverse Transcriptase (RTase), is an enzyme to synthesize cDNAs from RNA templates at a temperature range of 42℃ ~ 55℃ and shows the highest activity at 50℃. The high processivity and productivity of Thermo Reverse Transcriptase allows to amplify the high yield of product and can synthesize cDNA of target gene up to 12 kb from an RNA template.    Application Generation of the first-strand cDNA up to 12 kb for libraries or cloningTwo-step or One-step RT-PCR applications Conventional or real-time RT-PCR RT-PCR for detection of viral RNA     Figure 1. Comparison of activity of Thermo Reverse Transcriptase 200 u/㎕, 1,000 u/㎕ (= HelixCript™ Thermo Reverse Transcriptase) with other brand by RT-PCR for human gene. Total RNAs were extracted from human blood. The first-strand cDNA s were synthesized using total 700 ng RNA, oligo-d(T) primer, 200U HelixCript™Thermo Reverse Transcriptase at 50℃ or 200U Promega’s M-MLV RTase (Promega, USA) at 42℃. 10 % of cDNA reaction was used with primer set corresponding to fragment of human dynein gene and 2.5 U HelixAmp™ HyperSense-Taq DNA polymerase in 50 ul PCR reaction.Enzyme activities: RNA-dependent DNA polymerase. No RNase H activity  Recommended reaction temperature: 42 to 65°C  Storage buffer: 20mM Tris-HCl (pH7.5), 0.1mM EDTA-NaOH (pH 8.0), 100mM KCl, 0.01% Nonidet P-40, 1mM DTT, 50% Glycerol Purity: ≥90% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 200U enzyme and pUC19 plasmid at 37°C, 1hr)  RNase contamination test: Not detectable (Incubation with 200U enzyme and Human total RNA at 37°C, 1hr) Activity test in cDNA synthesis, RT-PCR and qRT-PCR: Correspond to reference (Human RNA target) Stability: 24 months at -20°C.