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1 u/㎕, 20 u/㎕
/Custom/BUDG1

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NanoHelix/Uracil DNA Glycosylase (UDG, UNG)
1 u/㎕, 20 u/㎕
/Custom/BUDG1

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BUDG1

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Uracil DNA Glycosylase (UDG, UNG)1 u/㎕, 20 u/㎕Removing uracil-containing DNAElimination of carryover contamination Engineered for heat susceptible Ideal enzyme for conventional and real-time PCR with dUTPProductsCat.No.ProductFeatureSizeBUDG1Uracil DNA Glycosylase1 u/㎕CustomBUDG10Uracil DNA Glycosylase20 u/㎕CustomDescriptionDataSpecificationDocumentsUracil DNA Glycosylase (UDG, UNG) is an engineered E. coli enzyme catalyzing the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. For PCR diagnosis, UDG can be used for efficient removal of carryover contaminated uracil-containing DNA. Application Prevention of carryover contamination Removing uracil-containing DNAGenotyping and Molecular Diagnosis Enzyme activities: Hydrolyzes uracil-glycosidic bonds in single- and double-stranded DNA Heat inactivation: +95°C for 5 minutes is sufficient for inactivation Storage buffer: 20mM Tris-HCl (pH8.0), 0.1mM EDTA-NaOH (pH 8.0), 50mM NaCl, 1mM DTT, 0.1ug/ul BSA, 50% Glycerol Purity: ≥90% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 10U enzyme and pUC19 plasmid at 37°C, 1hr) Activity test: Removal of uracil-containing PCR product with one unit of enzyme Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA