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1 u/㎕, 20 u/㎕
/Custom/BHLU20

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NanoHelix/Heat Labile UDG
1 u/㎕, 20 u/㎕
/Custom/BHLU20

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BHLU20

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NanoHelix

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产品说明

Heat Labile UDG1 u/㎕, 20 u/㎕Removing uracil-containing DNAHeat-labile, inactivated at over 40°C Ideal enzyme for conventional and real-time RT-PCR with dUTP ProductsCat.No.ProductFeatureSizeBHLU1Heat Labile UDG1 u/㎕CustomBHLU20Heat Labile UDG20 u/㎕CustomDescriptionDataSpecificationDocumentsHeat Labile UDG is a recombinant Uracil DNA glycosylase from marine bacterium BMTU 3346. UDG catalyzes the hydrolysis of the N-glycosidic bond between the uracil and sugar, leaving an apyrimidinic site in uracil-containing single or double-stranded DNA. Heat Labile UDG is fully active in the temperature range of 15 to 25℃ and inactivated at over 40℃. Due to this character, this enzyme is ideal for applying on the one-step RT-PCR system to remove the contaminated uracil-containing DNA in the reaction. Application Prevention of carryover contamination in RT-PCR Enzyme activities: Hydrolyzes uracil-glycosidic bonds in single- and double-stranded DNA. Heat inactivation: +50°C for 2 minutes are sufficient for inactivation. Storage buffer: 20mM Tris-HCl(pH7.8), 100mM KCl, 1mM DTT, 0.1mM EDTA, 0.05% Tween-20, 50% Glycerol Purity: ≥90% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 10U enzyme and pUC19 plasmid at 37°C, 1hr) Activity test: Removal of uracil-containing PCR product with one unit of enzyme Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA