首页 > NanoHelix > NanoHelix/RNase Inhibitor (Recombinant)
40 u/㎕
/Custom/BRNI

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NanoHelix/RNase Inhibitor (Recombinant)
40 u/㎕
/Custom/BRNI

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产品说明

RNase Inhibitor (Recombinant)40 u/㎕ ProductsCat.No.ProductFeatureSizeBRNIRNase Inhibitor (Recombinant)40 u/㎕CustomDescriptionDataSpecificationDocumentsRNase Inhibitor (recombinant), a recombinant protein of rat lung RNase inhibitor expressed in E. coli, effectively inactivates a broad spectrum of ribonuclease including RNase A, RNase B, and RNase T2. RNase inhibitor is useful to protect RNA from degradation by ribonucleases during cDNA synthesis, one-step RT-PCR, RNA synthesis, RNA preparation, and so on. Application cDNA synthesis / RT-PCRIn vitro transcription / In vitro translationRNA isolation and purification Enzyme activities: Inhibits enzymatic activity of RNases by noncovalently binding to the active site. Storage buffer: 20mM HEPES-KOH(pH7.6), 50mM KCl, 8mM DTT, 50% Glycerol Purity: ≥95% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 40U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 40U enzyme and Human total RNA at 37°C, 1hr) Activity test in RT-PCR and qRT-PCR: Correspond to reference (Human total RNA target) Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA