首页 > NanoHelix > NanoHelix/BstDNA polymerase, Large fragment8 u/㎕, 50 u/㎕/Custom/BBST8

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NanoHelix/BstDNA polymerase, Large fragment8 u/㎕, 50 u/㎕/Custom/BBST8

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BBST8

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产品说明

Bst DNA polymerase, Large fragment8 u/㎕, 50 u/㎕Thermophilic DNA polymerase Strong strand displacement activityLacks 5’ → 3’ exonuclease activityA recombinant protein, expressed in E. coli ProductsCat.No.ProductFeatureSizeBBST8Bst DNA polymerase, Large fragment8 u/㎕CustomBBST50Bst DNA polymerase, Large fragment50 u/㎕CustomDescriptionDataSpecificationDocumentsBst DNA polymerase, Large fragment is the portion of the Bacillus stearothermophilus DNA polymerase protein that contains the 5’ → 3’ polymerase activity but lacks 5’ → 3’ exonuclease activity. Bst DNA polymerase, Large fragment is purified from an E. coli strain as a recombinant. Application Loop-mediated isothermal amplification (LAMP)DNA strand displacement amplificationWhole genome amplification Enzyme activities: Modified to retain 5´ → 3´ polymerase activity while lacking 5´ →3´ exonuclease activity. Storage buffer: 10mM Tris-HCl (pH 7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA-NaOH (pH8.0), 0.1% Triton X-100, 50% Glycerol Recommended reaction temperature: 55 to 65°C Purity: ≥95% (SDS-PAGE) DNase contamination test: Not detectable (Incubation with 20U enzyme and pUC19 plasmid at 37°C, 1hr) RNase contamination test: Not detectable (Incubation with 20U enzyme and Human total RNA at 37°C, 1hr) Activity test: Correspond to reference (LAMP assay with λ phage DNA). Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA