首页 > NanoHelix > NanoHelix/Phi29DNA polymerase10 u/㎕, 100 u/㎕/Custom/BPHI100

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NanoHelix/Phi29DNA polymerase10 u/㎕, 100 u/㎕/Custom/BPHI100

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BPHI100

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NanoHelix

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产品说明

Phi29 DNA polymerase10 u/㎕, 100 u/㎕High fidelity DNA polymerase Strong strand displacement activityHigh processivity up to 70 kb Best enzyme for RCA of circular DNAEnzyme for whole genome amplificationProductsCat.No.ProductFeatureSizeBPHI10Phi29 DNA polymerase10 u/㎕CustomBPHI100Phi29 DNA polymerase100 u/㎕CustomDescriptionDataSpecificationDocumentsPhi29 DNA polymerase is a recombinant protein purified from E.coli cloned the gene encoding the DNA polymerase from Phi29 phage. Phi29 DNA polymerase is the replicative polymerase possessing the highest processivity and strand-displacement activity among the known DNA polymerase. ApplicationHighly accurate DNA synthesisRolling circle amplification (RCA)Multiple displacement amplification (MDA)Amplification of whole genome (WGA) Enzyme activities: Phi29 DNA polymerase is a highly processive polymerase featuring strong strand displacement activity which allows for highly efficient isothermal DNA amplification. Storage buffer: 10mM Tris-HCl (pH 7.5), 50mM KCl, 1mM DTT, 0.1mM EDTA-NaOH (pH8.0), 0.1% Triton X-100, 50% Glycerol Purity: ≥90% (SDS-PAGE) Activity test: Correspond to reference (WGA assay with human and plant genomic DNA) Stability: 24 months at -20°C

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA