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NanoHelix/Hot-Taq 2x Premix/Custom/BHPU

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BHPU

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NanoHelix

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产品说明

Hot-Taq 2x PremixConvenient ready-to-use 2x master mix format Chemically modified Taq DNA polymeraseAutomatic hot-start PCR enzymeConvenience of reactions set up at room temperatureApplied UDG system: prevention of carryover contaminationThe highest specificity of PCR amplificationProductsCat.No.ProductFeatureSizeBHPRHot-Taq 2x Premix-CustomBHPUHot-Taq 2x Premix, w/ UDG+ UDG SYSTEM*CustomDescriptionDataSpecificationDocumentsHot-Taq 2x Premix is an optimized mixture of Hot-Taq polymerase with reaction buffer and dNTPs as 2-fold concentration. Hot-Taq polymerase is a chemically-modified hot-start version of Taq DNA polymerase. This pre-mixed formulation is designed to save time and reduce error and contamination opportunities. Carryover contaminated PCR products could be removed by applying the UDG system. Application High specific amplification of DNA fragments SNP analysis and genotypingMultiplex PCR Amplification of high-GC or complex structured DNA Molecular diagnosis Properties: Conventional PCR, Carryover contamination prevention, Hot-start Heat activation: +95°C for 15 minutes Activity test: Performance test in PCR (Human genomic DNA, COMT, and p53 fragment): Corresponds to specification Stability: 12 months at -20°C.

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA