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NanoHelix/Ab+Taq 2x Premix/Custom/BAPU
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产品说明
Ab+Taq 2x PremixConvenient ready-to-use 2x master mix format Anti-Taq antibody complexed Taq DNA polymeraseAutomatic hot-start PCR enzymeFast enzyme activationConvenience of reactions set up at room temperatureApplied UDG system: prevention of carryover contaminationHigh specificity, sensitivity, and productivity ProductsCat.No.ProductFeatureSizeBAPRAb+Taq 2x Premix-CustomBAPUAb+Taq 2x Premix, w/ UDG+ UDG SYSTEM*CustomDescriptionDataSpecificationDocumentsAb+Taq 2x Premix is an optimized mixture of antibody-coupled Taq polymerase (Ab+ Taq polymerase) with reaction buffer and dNTPs as 2-fold concentration. This pre-mixed formulation is designed to save time and reduce error and contamination opportunities. Carryover contaminated PCR products could be removed by applying the UDG system.   Application Antibody-mediated Hot-start PCRHigh specific conventional and real-time PCRFast PCR assays with a short enzyme activation time and fast cycling protocols.Multiplex PCRSNP analysis and genotypingPCR for molecular diagnostics  Properties: Conventional PCR, Carryover contamination prevention, Hot-start Heat activation: +95°C for 2 minutes Activity test: Performance test in PCR using ABI Verity. (Human genomic DNA, POLD2 and TOPOII fragment): Corresponds to specification Stability: 12 months at -20°C 

NanoHelix热逆转录酶是M-MLV逆转录酶(RTase)的一种热稳定且RNase H阴性的变体,是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶,在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率,并且可以从RNA模板合成高达12 kb的靶基因cDNA。  


 


 


应用 


生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用 

常规或实时RT-PCR 

RT-PCR检测病毒RNA


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