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NanoHelix/One-Step RT-PCR Kit [Hot-Taq]/Custom/BORTH

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BORTH

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NanoHelix

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产品说明

One-Step RT-PCR Kit [Hot-Taq]Fast and easy one-tube setup Convenient enzyme mix and 2x reaction mixHeat-labile UDG system: prevention of carryover contaminationConvenience and reduced contamination riskThe highest specificity of PCR amplification ProductsCat.No.ProductFeatureSizeBORTHOne-Step RT-PCR Kit [Hot-Taq]-CustomBORTHUOne-Step RT-PCR Kit [Hot-Taq], w/UDG+ UDG SYSTEM*CustomDescriptionDataSpecificationDocumentsOne-Step RT-PCR Kit [Hot-Taq] is designed for specific and sensitive amplification of target genes in one-tube reaction from RNA templates. Thermo Reverse Transcriptase, Hot-Taq polymerase, and RNase Inhibitor are supplied as an enzyme mixture. An optimized buffer is provided as a 2x Reaction mix containing a buffer, MgCl2, and dNTPs as 2x concentration. Applied UDG system includes the Heal-labile UDG and dUTP. Application Viral RNA detectionGene-expression analysisMolecular DiagnosisEndpoint one-step RT-PCR from any RNA transcript Figure 1. Multiplex One-step RT-PCR of up to 4 targets by One-Step RT-PCR Kit [Hot-Taq]. Multiplex one-step RT-PCR in single-tube reaction was performed with total RNA extracted from hot pepper plant (Capsicum annuum) and by adding the indicated gene-specific primer sets. Each lane from 1 to 4 represents the progressive number of primer sets. The primer sets for the followed genes were used. CaAPX1 (374 bp), CaActin (461 bp), CaPF1 (615 bp), Cadhn (761 bp). Properties: Conventional RT-PCR, Hot-start Heat activation: +95°C for 15 minutes after reverse transcription reaction. Activity test: Performance test in PCR (Human total RNA, Actin fragment): Corresponds to specification Stability: 12 months at -20°C.

NanoHelix热逆转录酶是M-MLV逆转录酶（RTase）的一种热稳定且RNase H阴性的变体，是一种可在42℃〜55℃的温度下从RNA模板合成cDNA的酶，在50℃时显示最高的活性。热逆转录酶的高生产力和生产力可以扩增产物的高产率，并且可以从RNA模板合成高达12 kb的靶基因cDNA。

应用

生成长达12 kb的第一链cDNA用于文库或克隆

两步或一步RT-PCR应用

常规或实时RT-PCR

RT-PCR检测病毒RNA