OrderingItemCatalog #DescriptionQuantityPrice (USD)Plasmid104492Standard format: Plasmid sent in bacteria as agar stab1$75Add to CartAAV1104492-AAV1Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV8104492-AAV8Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV9104492-AAV9Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV Retrograde104492-AAVrgVirus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV PHP.eB104492-PHPeBVirus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartThis material is available to academics and nonprofits only.BackboneVector backboneAAV-Syn-FLEX (Search Vector Database)Backbone manufacturerScott SternsonBackbone sizew/o insert(bp)4894Total vector size (bp)6247Vector typeMammalian Expression, AAV, Cre/LoxGrowth in BacteriaBacterial Resistance(s)AmpicillinGrowth Temperature30°CGrowth Strain(s)NEB StableCopy numberLow CopyGene/InsertGene/Insert namejGCaMP7fAlt nameGCaMP3-T302L R303P A317L D380YAlt nameGCaMP3 variant 921Alt nameJanelia GCaMP7SpeciesR. norvegicus (rat), G. gallus (chicken); A. victoria (jellyfish)Insert Size (bp)1353PromoterSynapsinTag/ Fusion ProteinT7 epitope, Xpress tag, 6xHis Cloning InformationCloning methodRestriction Enzyme5′ cloning siteBsmBI(destroyed during cloning)3′ cloning siteBsmBI(destroyed during cloning)5′ sequencing primerACCACGCGAGGCGCGAGATAG(Common Sequencing Primers)Resource InformationTerms and LicensesUBMTAAncillary Agreement for Plasmids Containing FP MaterialsgenOway Notice of RIghtsIndustry TermsNot Available to IndustryInformation for AAV1 (Catalog # 104492-AAV1)(Back to top)PurposeReady-to-use AAV1 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA.Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV1PurificationIodixanol gradient ultracentrifugationBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsUsing FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.Information for AAV8 (Catalog # 104492-AAV8)(Back to top)PurposeReady-to-use AAV8 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA.Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV8PurificationIodixanol gradient ultracentrifugationBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsUsing FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.Information for AAV9 (Catalog # 104492-AAV9)(Back to top)PurposeReady-to-use AAV9 particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA.Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV9PurificationIodixanol gradient ultracentrifugationBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsUsing FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.Information for AAV Retrograde (Catalog # 104492-AAVrg)(Back to top)PurposeReady-to-use AAV Retrograde particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA.Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV preparations are suitable purity for injection into animals. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DeliveryVolume100 µLTiter≥ 7×10¹² vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)BufferPBS + 0.001% Pluronic F-68 + 200 mM NaClSerotypeAAV retrograde (AAVrg)PurificationIodixanol gradient ultracentrifugationBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsRetrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use. Using FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.Information for AAV PHP.eB (Catalog # 104492-PHPeB)(Back to top)PurposeReady-to-use AAV PHP.eB particles produced from pGP-AAV-syn-FLEX-jGCaMP7f-WPRE (#104492). In addition to the viral particles, you will also receive purified pGP-AAV-syn-FLEX-jGCaMP7f-WPRE plasmid DNA.Syn-driven, Cre-dependent GCaMP7f calcium sensor. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)BufferPBS + 0.001% Pluronic F-68SerotypePHPeB (plasmid #103005)PurificationIodixanol gradient ultracentrifugationBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsUsing FLEX vectors in vivo: LoxP sites in FLEX plasmids are known to recombine during DNA amplification and viral vector production, which may result in a minority of Cre-activated (i.e., "flipped") viral vectors. Addgene has measured this occurs in 0.01-0.03% of viral vectors in our typical production protocol. This can lead to a small number of cells exhibiting Cre-independent transgene expression in vivo. To address this, we recommend titrating to find the optimal AAV dosage required for Cre-dependent transgene expression and function in vivo. This may include reducing the viral vector dosage in order to reduce the likelihood of Cre-independent expression.Citation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.