OrderingItemCatalog #DescriptionQuantityPrice (USD)Plasmid105540Standard format: Plasmid sent in bacteria as agar stab1$75Add to CartAAV1105540-AAV1100 µL at titer ≥ 1×10¹³ vg/mLand Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV5105540-AAV5Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV9105540-AAV9Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV Retrograde105540-AAVrgVirus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV PHP.eB105540-PHPeB100 µL at titer ≥ 1×10¹³ vg/mLand Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartThis material is available to academics and nonprofits only.BackboneVector backbonepAAV (Search Vector Database)Vector typeMammalian Expression, AAV, Cre/LoxGrowth in BacteriaBacterial Resistance(s)AmpicillinGrowth Temperature37°CGrowth Strain(s)NEB StableCopy numberUnknownGene/InsertGene/Insert nameEGFP-CrePromoterhSynCloning InformationCloning methodUnknownResource InformationSupplemental DocumentspENN.AAV.hSyn.HI.GFP-Cre.WPRE.SV40 (p1848).gbTerms and LicensesUBMTAAncillary Agreement for Plasmids Containing FP MaterialsIndustry TermsNot Available to IndustryArticle Citing this Plasmid1 ReferenceDepositor CommentsPenn Vector Core number p1848Information for AAV1 (Catalog # 105540-AAV1)(Back to top)PurposeReady-to-use AAV1 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA.Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging PlasmidsEncode adenoviral helper sequences and AAV rep gene, AAV1 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV1PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV5 (Catalog # 105540-AAV5)(Back to top)PurposeReady-to-use AAV5 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA.Expression of EGFP-Cre from hSyn promoter. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 7×10¹² vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap geneBufferPBS + 0.001% Pluronic F-68 + 200 mM NaClSerotypeAAV5PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV9 (Catalog # 105540-AAV9)(Back to top)PurposeReady-to-use AAV9 particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA.Synapsin-driven EGFP-Cre expression. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV9PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsData submitted about 105540-AAV9 by requesting scientist(s):Data 1: Mouse, Brain parenchymaInformation for AAV Retrograde (Catalog # 105540-AAVrg)(Back to top)PurposeReady-to-use AAV Retrograde particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA.Expression of EGFP-Cre from hSyn promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 7×10¹² vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV retrograde cap gene from rAAV2-retro helper (plasmid #81070)BufferPBS + 0.001% Pluronic F-68 + 200 mM NaClSerotypeAAV retrograde (AAVrg)PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsRetrograde functionality is dependent on high viral titers. Addgene recommends not diluting your AAV preps prior to use.Information for AAV PHP.eB (Catalog # 105540-PHPeB)(Back to top)PurposeReady-to-use AAV PHP.eB particles produced from pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 (#105540). In addition to the viral particles, you will also receive purified pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 plasmid DNA.Expression of EGFP-Cre from hSyn promoter. These AAV were produced with the PHPeB serotype, which permits efficient transduction of the central nervous system. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, PHP.eB cap gene pUCmini-iCAP-PHP.eB (plasmid #103005)BufferPBS + 0.001% Pluronic F-68SerotypePHPeB (plasmid #103005)PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Addgene CommentsCitation Information: When using the PHP.eB serotype in future publications, please acknowledge Viviana Gradinaru and cite Chan et al., Nat Neurosci, 20(8):1172-1179. Pubmed.