首页 > Addgene > Addgene/pWZL Neo Myr Flag AMHR2/1unit/20424

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Addgene/pWZL Neo Myr Flag AMHR2/1unit/20424

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OrderingItemCatalog #DescriptionQuantityPrice (USD)Plasmid20424Standard format: Plasmid sent in bacteria as agar stab1$75Add to CartThis material is available to academics and nonprofits only.BackboneVector backbonepWZL-Neo-Myr-Flag-DEST (Search Vector Database)Backbone manufacturerWilliam Hahn Lab (available at Addgene, #15300)Vector typeMammalian Expression, RetroviralSelectable markersNeomycin (select with G418)Growth in BacteriaBacterial Resistance(s)AmpicillinGrowth Temperature37°CGrowth Strain(s)Stbl3Growth instructionsStbl3 cells to prevent recombination.Copy numberUnknownGene/InsertGene/Insert nameAMHR2Alt nameanti-Mullerian hormone receptor, type II isoform 3SpeciesH. sapiens (human)MutationC473G mutation when compared to Genbank reference sequence NP_001158163.1GenBank IDEntrez GeneAMHR2(a.k.a. AMHR, MISR2, MISRII, MRII)Tags/ Fusion ProteinsMyr (N terminal on backbone)Flag (N terminal on backbone)Cloning InformationCloning methodGateway Cloning5′ sequencing primerpWZL-Fwd (GAA CCT CCT CGT TCG ACC)3′ sequencing primerpWZL-Rev (TTC CGG GCC CTC ACA TTG)(Common Sequencing Primers)Resource InformationA portion of this plasmid was derived from a plasmid made by2-B2Terms and LicensesUBMTAIndustry TermsNot Available to IndustryDepositor CommentsORFs were cloned into Gateway compatible pEntry vectors. An LR recombination reaction was performed to move the ORF to pWZL-Neo-Myr-Flag DEST such that the ORF would have 5' myristoylation and flag tags.This plasmid is part of a kinase library, and the gene has been verified, but has not been fully sequenced for minor mutations.Please see the following link for plasmids from this article that are not part of the kinase libraryhttp://www.addgene.org/pubmed/17574021

Addgene腺相关病毒（AAV）是最初被发现为腺病毒原种污染物的小型病毒。使用AAV进行研究的一个主要优点是它受复制限制，通常不引起人类疾病。由于这些原因，AAV通常以较低的生物安全水平被包含并且在体内引起相对较低的免疫学作用。虽然AAV可以在BSL-1处理，但表达癌基因或毒素的AAV应该在BSL-2处理。

AAV可以以低免疫应答和低毒性转导分裂和非分裂细胞。尽管重组AAV不能整合到宿主基因组中，但转基因表达可以长期存在。目前，AAV的实用性受到其小包装容量（包括ITR在内约4.5 kb）的限制，尽管有很多兴趣和精力来扩大这种容量。

传统上，AAV需要存在另一种“辅助”病毒（例如腺病毒或疱疹病毒）才能传播。这是由于AAV对某些介导AAV复制的外源基因产物的依赖。“无辅助病毒的系统”已经绕过了这一要求，该系统无需使用辅助病毒就可以生产感染性AAV颗粒。替代地，可以在AAV生产过程中通过辅助质粒（例如pHelper）和特异性包装细胞系（例如HEK293细胞）提供特异性基因产物。