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Addgene/pAAV.CMV.PI.EGFP.WPRE.bGH/1unit/105530-AAV5
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OrderingItemCatalog #DescriptionQuantityPrice (USD)Plasmid105530Standard format: Plasmid sent in bacteria as agar stab1$75Add to CartAAV1105530-AAV1Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV2105530-AAV2Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV5105530-AAV5Virus (100 µL at titer ≥ 7×10¹² vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV8105530-AAV8Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV9105530-AAV9Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartAAV rh10 (similar to AAV10)105530-rh10Virus (100 µL at titer ≥ 1×10¹³ vg/mL)and Plasmid.More Information123456789101112131415161718192021222324252627282930$380Add to CartThis material is available to academics and nonprofits only.BackboneVector backbonepAAV (Search Vector Database)Vector typeMammalian Expression, AAVGrowth in BacteriaBacterial Resistance(s)AmpicillinGrowth Temperature37°CGrowth Strain(s)NEB StableCopy numberUnknownGene/InsertGene/Insert nameEGFPPromoterCMVCloning InformationCloning methodUnknown5′ sequencing primerCMV-F(Common Sequencing Primers)Resource InformationSupplemental DocumentspAAV.CMV.PI.EGFP.WPRE.bGH (p0101).gbTerms and LicensesUBMTAAncillary Agreement for Plasmids Containing FP MaterialsIndustry TermsNot Available to IndustryArticle Citing this Plasmid1 ReferenceDepositor CommentsPenn Vector Core number p0101Information for AAV1 (Catalog # 105530-AAV1)(Back to top)PurposeReady-to-use AAV1 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV1 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV1PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV2 (Catalog # 105530-AAV2)(Back to top)PurposeReady-to-use AAV2 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 7×10¹² vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV2 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV2PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV5 (Catalog # 105530-AAV5)(Back to top)PurposeReady-to-use AAV5 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 7×10¹² vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV5 cap geneBufferPBS + 0.001% Pluronic F-68 + 200 mM NaClSerotypeAAV5PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV8 (Catalog # 105530-AAV8)(Back to top)PurposeReady-to-use AAV8 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV8 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV8PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV9 (Catalog # 105530-AAV9)(Back to top)PurposeReady-to-use AAV9 particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAV9 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAV9PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.Information for AAV rh10 (similar to AAV10) (Catalog # 105530-rh10)(Back to top)PurposeReady-to-use AAV rh10 (similar to AAV10) particles produced from pAAV.CMV.PI.EGFP.WPRE.bGH (#105530). In addition to the viral particles, you will also receive purified pAAV.CMV.PI.EGFP.WPRE.bGH plasmid DNA.CMV-driven EGFP control. These AAV preparations are suitable purity for injection into animals.DeliveryVolume100 µLTiter≥ 1×10¹³ vg/mLPricing$350 USD for preparation of 100 µL virus + $30 USD for plasmid.StorageStore at -80℃. Thaw just before use and keep on ice.ShipmentViral particles are shipped frozen on dry ice. Plasmid DNA (≥ 200ng) will also be included in the shipment.Viral Production & UsePackaging Plasmidsencode adenoviral helper sequences and AAV rep gene, AAVrh10 cap geneBufferPBS + 0.001% Pluronic F-68SerotypeAAVrh10PurificationIodixanol gradient ultracentrifugationReporter GeneEGFPBiosafetyRequestor is responsible for compliance withtheir institution"s biosafety regulations.Lentivirus is generally considered BSL-2. AAV isgenerally considered BSL-1, but may requireBSL-2 handling depending on the insert.Biosafety GuideResource InformationTerms and LicensesAncillary Agreement for Penn VectorsTerms of Use for Viral VectorsIndustry TermsNot Available to IndustryViral Quality ControlQuality Control: Addgene ensures high quality viral vectors by optimizing and standardizing production protocols and performing rigorous quality control (QC) (see a list of our QC assays). Thespecific QC assays performed varies for each viral lot. To learn which specific QC assays were performed on your lot, please contact us.Titer: the exact titer of your sample will be reported on the tube. The titer you see listed on this page is the guaranteed minimum titer. See how titers are measured.Visit our viral production page for moreinformation.

Addgene腺相关病毒(AAV)是最初被发现为腺病毒原种污染物的小型病毒。使用AAV进行研究的一个主要优点是它受复制限制,通常不引起人类疾病。由于这些原因,AAV通常以较低的生物安全水平被包含并且在体内引起相对较低的免疫学作用。虽然AAV可以在BSL-1处理,但表达癌基因或毒素的AAV应该在BSL-2处理。


AAV可以以低免疫应答和低毒性转导分裂和非分裂细胞。尽管重组AAV不能整合到宿主基因组中,但转基因表达可以长期存在。目前,AAV的实用性受到其小包装容量(包括ITR在内约4.5 kb)的限制,尽管有很多兴趣和精力来扩大这种容量。


传统上,AAV需要存在另一种“辅助”病毒(例如腺病毒或疱疹病毒)才能传播。这是由于AAV对某些介导AAV复制的外源基因产物的依赖。“无辅助病毒的系统”已经绕过了这一要求,该系统无需使用辅助病毒就可以生产感染性AAV颗粒。替代地,可以在AAV生产过程中通过辅助质粒(例如pHelper)和特异性包装细胞系(例如HEK293细胞)提供特异性基因产物。


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