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JPT/Multiwell RepliTope™ Human (NY-ESO-1)/RT-MW-0016/
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MultiwellRepliTope™Human(NY-ESO-1)ProductCode:RT-MW-0016Peptidemicroarraydisplaying44peptidesin21subarrays(3copies/subarray)forincubationwith21samplesinparallelusinganArraySlide24-4chamber.Apeptidescan(15merswith11aaoverlap)throughCancer/testisantigen1(NY-ESO-1)(Swiss-ProtID:P78358)ofHomosapiens(Human)isdisplayed.Amount:1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide)Purity:FreeoftruncatedsequencesDeliveryFormat:Peptidesimmobilizedontoglassslide(3x1inches;75by25mm)Application(s):Immunemonitoring,Humoralimmuneresponse,SEROMarkerdiscovery,Antibodyepitopediscovery,BindingexperimentsIndication(s)/Topic(s):Cancer,Testis/ovarycancerDeliveryTime:10-12daysFortheincubationofMultiwellRepliTope™MicroarrayswithdifferentsamplesanincubationchambersuchasArrayslidechamber24-4isneeded!JPT"sRepliTope™MultiwellPeptideMicroarraysJPTPeptideTechnologiesappliesitsuniquepeptidemicroarrayplatformtogeneratepeptidemicroarraysonglassslidesforbiomarkerdiscovery,immunemonitoring,detectionandvalidationofproteininteractions.OverlappingpeptidesareimmobilizedonglassslidesviaaflexIBLelinkerbychemoselectivecoupling.Thecovalentlyattachedpeptidesaredisplayedinthreecopiespersubarrayoneachslide.The21subarrayscanbeincubatedwithdifferentsamplesusinganArrayslidechamber24-4andregularELISAequipment(washer,incubator).Read-outviafluorescenceresultsinlowbackgroundandhighsensitivity.BenefitsofMultiwellRepliTope™PeptideMicroarrays-Incubateseveraldifferentsamplesononemicroarrayslide-Gethundredsofidenticalmicroarraycopies-Peptidesarefreeoftruncatedsequences-Smallamountsofyourprecioussamplesareneededforincubation-ApplicabletoavarietyofBIOLOGicalsamples(e.g.serum,plasma,antibody)-Lowbackgroundonglasssurface-Read-outviafluorescenceMultiwellRepliTope™Human(NY-ESO-1)SelectedReferencesforJPT"sPeptideMicroarrays"StructureofGPN-loopGTPaseNpa3andImplicationsforRNAPolymeraseIIAssembly"Niessertal.,MolCellBiol. (2015)-PMID:26711263"ProtectiveEfficacyofAdenovirus-proteinVaccinesAgainstSIVChallengesinRhesusMonkeys"Barouchetal.,Science(2015)-PMID:26138104"IdentificationandCharacterizationofCommonBCellEpitopeinBovineLeukemiaVirusviaHigh-throughputPeptideScreeningSysteminInfectedCattle"Baietal.,Retrovirology(2015)-PMID:26715158"High-ThroughputMicroarrayIncubationsUsingMulti-WellChambers"Zerwecketal.,MethodsMolBiol.(2015)-PMID:26490464"PeptideMicroarraysforCoverageofSequenceDiversityinMonitoringtheBcellImmuneResponse(TECH2P.911)"Castro,etal.,TheJournalofImmunology(2015)-PMID:n.a."AntibodiestoInfluenzaNucleoproteinCross-reactwithHumanHypocretinReceptor2"Ahmedetal.,ScienceTranslationalMedicine(2015)-PMID:26136476"SerumReactomeInducedbyBordetellaPertussisInfectionandPertussisVaccines:QualitativeDifferencesinSerumAntibodyRecognitionPatternsRevealedbyPeptideMicroarrayAnalysis"Valentinietal.,BMCImmunol.(2015)-PMID:26129684ApplicationNotes"AModularApproachforEpitopeDiscoveryandHigh-ResolutionProfilingofHumoralImmuneResponses"Pawlowskietal.(2013)Fulltext"MultipleSclerosisandEpsteinBarrVirusInfection.AnEpitopeMappingStudy"Reimeretal.(2014)Fulltext"TheChallengeofAntigenSequenceDiversity:SolutionswithULTRAPeptideLibraries"Pawlowskietal.(2015)Fulltext"RapidMimotopeOptimizationforPharmacokineticAnalysisoftheNovelTherapeuticantibodyIMAB362"Danescharetal.(2014)FulltextTestimonialsforJPT"sPeptideMicroarrays"Overthepastfiveyearsmyresearchteamhasbeenactiveinthedevelopmentofspecies-specificpeptidearrays.OurABIlitytoadvancethistechnologyintonewresearchareashasbeendependentonworkingwiththehighestqualityofarrays.Inthisregard,JPT"scommitmenttoexcellencehasbeenacriticalfoundationforthesuccessofourresearchprogram.Ihavebeenconsistentlyimpressedwiththeircustomerservice,technicalexpertiseandprofessionalism.Whenitcomestopeptideworktherearenootheroptionsforourgroup,JPTisthebest."ScottNapper,PhD,VaccineandInfectiousDiseaseOrganization(VIDO),UniversityofSaskatchewan,Canada"TheRV144HIVtrialisconsideredasoneoffirstsuccessfulHIVvaccinetrials.IthasbecomeclearthattheV2loopofgp120isanimportantsiteforimmunogenicityandprotectionfromHIVinfection.TheuseofJPT"sPepStar™microarraytechnologyhasbeenveryusefulforthecorrelationoftheclincialoutcomewithhumoralimmuneresponses.AshavethecyclicpeptidesbeenfromJPTtovalidatethesefindings!"JeffCurrier,PhD,WalterReedArmyInstitute,Rockville,Maryland,USA"WestudymodulationofdopaminergicneurotransmissonbyTAAR1andD2R.ThefunctionalinteractionofthetworeceptorsinthebrainsupportsTAAR1asatargetforthetreatmentofpsychiatricdisorderssuchasschizophreniaorbipolardisorder.Tomaptheepitopesofournewlygeneratedspecificanti-ratTAAR1antibodiesweusedJPT"sPepStar™peptidemicroarrays.Thepeptidemicroarraysgreatlycontributedtooursuccessfulandrecentlypublishedstudy.WewereverysatisifiedwiththeexceptionalproductandservicedeliveredbyJPTPeptideTechnologiesaswellastheirscientificCustomerSupportwhichwasalwaysatourdisposal."StefanObermüller,F.HoffmannLaRocheLtd.,RochePharmaResearchandEarlyDevelopment,Basel,SwitzerlandMultiwellRepliTope™Human(NY-ESO-1)TechnicalData:ApplicationAntibodyepitopediscoveryCancerbiomarkerresearchImmunemonitoringProteinNameCancer/testisantigen1(NY-ESO-1)OrganismHomosapiens(Human)Indication/TopicCancerMalignantgenitalcancersNumberofpeptides44 FurtherInformation:Amount1peptidemicroarray(eachpeptideisdeposited3xpersubarray,21subarraysperslide)SpecificationsPeptidescan(15merswith11aaoverlap)DocumentationCDwithProtocol,Datasheet,gal-fileandgal-viewerSwiss-ProtIDP78358NCBINP_001318SequenceMQAEGRGTGGSTGDADGPGGPGIPDGPGGNAGGPGEAGATGGRGPRGAGAARASGPGGGAPRGPHGGAASGLNGCCRCGARGPESRLLEFYLAMPFATPMEAELARRSLAQDAPPLPVPGVLLKEFTVSGNILTIRLTAADHRQLQLSISSCLQQLSLLMWITQCFLPVFLAQPPSGQRRProteinLength(aa)180
JPT的关键技术平台基于该公司强大而长期的技术,化学和生物学背景。JPT已经开发了几种独特的方法,可解决细胞疗法和疫苗开发过程的所有阶段,包括生物标记物发现,新表位优先,免疫监测和疫苗功效测试。
此外,这四个技术平台为靶向蛋白质组学和分子诊断奠定了基础。JPT的技术在需要进行肽筛查的领域中得到了广泛的应用。典型示例包括:筛选蛋白肽,酶活性(蛋白酶,激酶,磷酸酶,甲基转移酶)或肽前导结构的系统优化。
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