首页 > Hycult Biotech > Hycult Biotech/MBL-C, Mouse, ELISA kit/HK209-02/2 x 96 det.

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Hycult Biotech/MBL-C, Mouse, ELISA kit/HK209-02/2 x 96 det.

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HK209-02

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Description:MBL-C,Mouse,ELISAkitMannoseBindingLectin(MBL)alsocalledmannose-ormannan-bindingprotein(MBP)isamemberofthecollectinsandanimportantelementininnateimmunity.MBLisanoligomericlectinthatrecognizescarbohydratesasmannoseandN-acetylglucosamineonpathogens.MBLcontainsacysteinerich,acollagenlikeandacarbohydraterecognitiondomain.ItformsacomplexwithC1r/C1slikeserineproteasesdesignatedMASPthatproteolyticallycleaveC4,C2andC3.MBLisabletoactivatethecomplementpathwayindependentoftheclassicalandalternativecomplementactivationpathways.TheMBL-MASPpathway(betterknownasthelectinpathway)isantibodyandC1q-independent.MBLexhibitscomplement-dependentanti-bacterialactivityandactsdirectlyasanopsonicandthereforeplaysanimportantroleininnateimmunity.MBLissynthesizedbyhepatocytesandhasbeenisolatedfromtheliverandserumofseveralvertebratespecies.OnlyoneformofhumanMBLhasbeencharacterized,whiletwoformsarefoundinrhesusmonkeys,rabbits,ratsandmice.ThemurineformsareknownasMBL-AandMBL-C.TheMBL-Cconcentrationsinserumareabout6-foldhighercomparedtothatofMBL-A.MBL-A,butnotMBL-CwasfoundtobeanacutephaseproteinincaseinandLPS-injectionmodels.MBL-CexistsinhigheroligomericformsthanMBL-A.InplasmaofhealthymiceMBL-Cconcentrationsrangefrom16to118µg/ml.IninfectiousdiseasestheMBL-Cconcentrationwasnotfoundtoincreasesignificantly.Specifications:CatalognumberHK209-02ProducttypeAssaysQuantity2x96det.StandardrangeThemeasurableconcentrationrangeis3.1-200ng/ml.DetectionlevelTheminimumconcentrationwhichcanbemeasuredismouse3.1ng/mlmouseMBL-C.Workingvolume100µl/wellSpeciesMouseApplicationThemouseMBL-CELISAkitistobeusedfortheinvitroquantitativedeterminationofmouseMBL-Cinserum,plasmaorcellculturesamples.DiseaseAutoimmunity,GastroenterologyPrincipleThemouseMBL-CELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedmouseMBL-C.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofcitricacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthemouseMBL-Cstandards(log).ThemouseMBL-Cconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve.StorageandstABIlityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.References1.Vriesde,Betal;Themannose-bindinglectinpathwayisinvolvedincomplementactivationinthecourseofrenalischemia-reperfusioninjury.AmJPathol2004,165:16772.Trouw,Letal;Activationofthelectinpathwayinmurinelupusnephritis.MolImmunol2005,42:731

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