首页 > Hycult Biotech > Hycult Biotech/TNF-alpha, Human, ELISA kit/HK307-02/2 x 96 det.

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Hycult Biotech/TNF-alpha, Human, ELISA kit/HK307-02/2 x 96 det.

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Description:TNF-alpha,Human,ELISAkitTumornecrosisfactoralpha(TNF-alpha),ahomotrimeric17KDprotein,isapotentmediatorofinflammatoryandmetabolicfunctions.TNF-alphawasoriginallydetectedasahighlycytotoxiccytokinefortumorcells,itcausestumornecrosisinvivoandshowscytolyticactivityagainsttumorcellsinvitro.FurThermore,TNF-alphahasbeenimpliedascentralmediatorinshockinducedbygramnegativemicro-organisms.ThecytokineTNFisfoundtobeacentralmediatorinmanyinflammatoryandimmunologicalprocesses:itcanbeinducedbyvariousproductsofmicro-organismsandbyvariouscytokinesbutitalsoinducesonitsturntheproductionofmanycytokines.SignaltransductionoccursviatwotypesofTNF-receptors,thep55(RI)andp75(RII)TNF-receptors.Thereceptorsdifferstronglyintheirintra-cellularsignalingpathways.TheTNF-alphatrimerinteractswitheitherofthetwotypesofTNF-RleADIngtoreceptorcross-linking.Specifications:CatalognumberHK307-02ProducttypeAssaysQuantity2x96det.Standardrange8-2,000pg/mlDetectionlevel10pg/mlWorkingvolume100µl/wellSpeciesHumanApplicationThehumanTNF-αELISAkitistobeusedfortheinvitroquantitativedeterminationofhumanTNF-αinserum,plasma,andcellculturesupernatantsamples.DiseaseAutoimmunity,GastroenterologyPrincipleThehumanTNF-alphaELISAisaready-to-usesolid-phaseenzyme-linkedimmunosorbentassaybasedonthesandwichprinciplewithaworkingtimeof3½hours.Theefficientformatof2plateswithtwelvedisposable8-wellstripsallowsfreechoiceofbatchsizefortheassay.Samplesandstandardsarecapturedbyasolidboundspecificantibody.BiotinylatedtracerantibodywillbindtocapturedhumanTNF-alpha.Streptavidin-peroxidaseconjugatewillbindtothebiotinylatedtracerantibody.Streptavidin-peroxidaseconjugatewillreactwiththesubstrate,tetramethylbenzidine(TMB).Theenzymereactionisstoppedbytheadditionofcitricacid.Theabsorbanceat450nmismeasuredwithaspectrophotometer.Astandardcurveisobtainedbyplottingtheabsorbance(linear)versusthecorrespondingconcentrationsofthehumanTNF-alphastandards(log).ThehumanTNF-alphaconcentrationofsamples,whicharerunconcurrentlywiththestandards,canbedeterminedfromthestandardcurve.StorageandstABIlityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.References1.Molnar,Letal;DetectionofTNFalphaexpressioninthebonemarrowanddeterminationofTNFalphaproductionofperipheralbloodmononuclearcellsinmyelodysplasticsyndrome.PatholOncolRes2000,6:182.Younis,Aetal;Staphylococcusaureusleucocidin,avirulencefactorinbovinemastitis.JDairyResearch2005,72:188

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