Description:IFN-alpha,Mouse,mAbF18,functionalstudyantibody,lowendotoxinThemonoclonalantibodyF18recognizesandneutralizesbothnaturalandrecombinantmousealphaInterferon(IFN-α).IFN-αisacytokinethatbelongstothetypeIinterferons(IFN-I).IFN-αissecretedbymanycelltypesincludinglymphocytes(NKcells,B-cellsandT-cells),macrophages,fibroblasts,endothelialcells,osteoblasts,microgliaandothers.InterferonsstimulatebothmacrophagesandNKcellstoelicitananti-viralresponse,andarealsoactiveagainsttumors.AlthoughallcellscanproduceIFN-I,plasmacytoiddendriticcells(pDCs)produce1,000-foldhigherlevelsthanothercelltypes,andareresponsIBLeforsystemicIFN-Iresponsestomanyviruses.TheyarecoinedasthenaturalIFN-producingcells.However,underdeprivedpDCcondition,otherdendriticcellsarecapableofproducinghighlevelsofIFN-I.InterferonswereinitiallycharacterizedfortheirABIlityto‘interfere"withviralreplication,slowcellproliferation,andprofoundlyalterimmunity.IFN-αhasseveralregulatoryrolesanddiverseBIOLOGicalactivities,includingcontrolofcellularandhumoralimmuneresponses,inflammation,andtumorregression.Inaddition,IFN-αparticipatesintheregulationofvariouscellularandhumoralprocessessuchastheendocrinesystemmodulatesbehavior,brainactivity,temperature,glucosesensitiveneurons,feedingpatternandopiateactivity.WiththeavailabilityofmonoclonalantibodiesdirectedagainstIFN-α,itispossibletointerpretresultsobtainedfromcrudematerialscontainingbothIFN-αandIFN-β.Thedifficultiesinstudyinginvitroandinvivoeffectsof‘type1’InterferonsarisefromthefactthatbothalphaandbetaInterferonsareproducedinresponsetothesamestimuliandalsoseemtoactviathesamereceptor.TheseInterferonactivitiescanonlybedistinguishedfromoneanotherbyuseofspecificneutralizingantibodies.Specifications:CatalognumberHM1001-FSProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay.ApplicationFlowcytometry,Functionalstudies,Immunoassays,WesternblotApplicationNotesFC:ForintracellularstainingofIFN-α,cellscanbefixedin1%formaldehyde;blockedandpermeabilizedin0.2%saponin,5%normalrabbitserumfor30minutesonice.(Ref.2)FS:NeutralizationofIFN-αbyadding1μgantibodyF18permousei.v.beforetreatmentwith35μgLPSi.p.,decreasedtheLPS-inducedIL-1βserumresponse.(Ref.3)./br>IA:HM1001hasbeenusedasacaptureantibodytodeterminespecificity.UseForflowcytometrydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:10.Beforeuseinbiologicalassays,theproductmustbefiltersterilizedanddependingontheconcentrationtobeuseddialyzedagainstculturemediumtoremovethesodiumazideadded.ForneutralisationofbiologicalactivitydilutionshavetobemadeaccordingtotheamountofInterferontobeinactivated.ThecrossreactivitywithHumanIFN,MouseIFN-gammaandMouseIFN-betawasnegative.IsotypeRatIgG1SpeciesMouseCrossreactivityHuman-NoAliasIFN-αPositiveControlPlasmacytoiddendriticcellsReferences1.Dalod,Metal;Interferonα/βandinterleukin12responsestoviralinfections:pathwaysregulatingdendriticcellcytokineexpressioninvivo.JExpMed2002,195:5172.Diebold,Setal;Viralinfectionswitchesnonplasmacytoiddendriticcellsintohighinterferonproducers.Nature2003,424:3243.Joshi,Vetal;AroleforStat1intheregulationoflipopolysaccharide-inducedInterleukin-1βexpression.JInterferonCytokineRes2006,26:739.4.Wilkstrom,Metal;AChemokine-LikeViralProteinEnhancesAlphaInterferonProductionbyPlasmacytoidDendriticCellsbutDelaysCD8TCellActivationandImpairsViralClearance.JVirol2013,87:7911StorageandstabilityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseInfectiousdiseases,Tumorimmunology