首页 > Hycult Biotech > Hycult Biotech/TLR3, Human, mAb TLR3.7, functional study antibody, low endotoxin/HM2096-FS/0.5 mg

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Hycult Biotech/TLR3, Human, mAb TLR3.7, functional study antibody, low endotoxin/HM2096-FS/0.5 mg

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HM2096-FS

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Description:TLR3,Human,mAbTLR3.7,functionalstudyantibody,lowendotoxinThemonoclonalantibodyTLR3.7recognizesthe116kDahumanToll-likereceptor3(TLR3,CD283).Toll-likereceptors(TLRs)arehighlyconservedfromDrosophilatohumansandsharestructuralandfunctionalsimilarities.TLRsconstituteofafamilyofpatternrecognitionreceptors(PRRs)thatmediatecellularresponsestoalargevarietyofpathogens(viruses,bacteria,andparasites)byspecificrecognitionofso-called‘pathogen-associatedmolecularpatterns’.ActivationofTLRs,afamilyofatleast11differentmembersthatfunctioneitherashomo-orheterodimers,leadstoactivationofNFκBDependentandIFN-regulatoryfactor-dependentsignalingpathways.TLRshaveacentralroleininnateimmunityandarealsorequiredforthedevelopmentofanadaptiveimmuneresponse.TLRsareexpressedbyvariouscellsoftheimmunesystem,suchasmacrophagesanddendriticcells.TLRsareclassIreceptors,withasingleα-helixthatspansthecellmembrane.Theyrecognizeandrespondtomoleculesderivedfrombacterial,viralandfungalpathogens,suchaslipopolysaccharide(LPS)fromtheoutermembraneofGramnegativebacteria,peptidoglycanfragmentsfrombacterialcellwallsandsingle-strandedanddouble-strandedRNAfromviruses.SomeformsofRNAandDNAfrompathogensexhibitimmutablefeaturesthatdistinguishthemfromnucleicacidsofhigherorganisms.Forexample,dsRNA,isacommonintermediateofviralreplicationandapotentindicatorofinfection.Toll-likereceptor3(TLR3)recognizesviraldouble-strandedRNAanditssyntheticanalogpolyriboinosinic:polyribocytidylicacid(poly(I:C)).TLR3isnormallylocatedinacidicendosomeswhereitsluminalectodomain(ECD)encountersdsRNAandinducestypeIinterferon(IFN),inflammatorycytokine/chemokineproductionanddendriticcell(DC)maturationviatheadaptorproteinTICAM-1(alsocalledTRIF).BasedonthedifferentsubcellularlocalizationofcytosolicRNAreceptorsandTLR3,thesereceptorsseemtoplaydistinctrolesinanti-viralimmuneresponses.Specifications:CatalognumberHM2096-FSProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay.ApplicationFlowcytometry,Frozensections,Functionalstudies,Immunofluorescence,Immunoprecipitation,ParaffinsectionsApplicationNotesF:driedsections,fixedwith4%paraformaldehydeandsubsequentlywashedinPBSandMQ.Sectionswerequenchedwith0.3%H2O2inmethanolandwashedinPBS.SectionswerepermeABIlizedwith0.4%triton-X100inPBS.Pretreatedslideswereblockedwith1%horseserumfor20’andincubatedo/nwithantibody(Ref.5).FC:cellswereincubatedwith1μgantibodytogetherwith10μghumanIgGfor30’at4°CinPBS/0,5%BSA(Ref.3).FS:7.5*104MRC5cellswerepre-treatedwith10-20μg/mlfor1-24hat37°C.MonoclonalantibodyTLR3.7inhibitsdsRNA-inducesIFN-betaproduction(Ref.1).IF:Cytospinsofmonocyte-derivediDCswerefixedfor30’with3%formaldehydeinPBS,permeabilizedwithPBS/1%BSA/0.5%saponin.AfterPBSwashslideswereincubatedfor1hatRTwith20ug/mlantibodyinPBS/1%BSA(Ref.3).P:Formalinfixed,paraffinembeddedsectionsweredeparaffinizedwithxylene,followedbywashesin95%and70%EtOH.Sectionswerewashedwithwaterandpermeabilizedwith0.4%triton-X100inPBS.Pretreatedslideswereblockedwith1%horseserumfor20’andincubatedo/nwithantibody(Ref.5).W:Totalcellularproteinwasloadedon7.5%SDS-PAGEandblottedonPDVF.Blotswereincubatedwith2μg/mlantibodyo/nat4°C(Ref.6).UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenHumanFlag-taggedTLR3stablyexpressedbyBa/F3cellsIsotypeMouseIgG1SpeciesHumanCrossreactivityCanine-Yes,Mouse-YesAliasCD283,Toll-likereceptor3Genename:TLR3PositiveControlMonocytes,granulocytes,lymphocytes,humanfibroblast,MRC-5&FS-4cellsNegativeControlHEK293cellsReferences1.Matsumoto,Metal;EstablishmentofamonoclonalantibodyagainsthumanToll-likereceptor3thatblocksdouble-strandedRNA-mediatedsignalling.BiochemBiophysResCommun2002,293:13642.Oshiumi,Hetal;TICAM-1,anadaptormoleculethatparticipatesinToll-likereceptor3-mediatedinterferon-betainduction.NatImmunol2003,4:1613.Matsumoto,Metal;SubcellularlocalizationofToll-likereceptor3inhumandendriticcells.JImmunol2003,171:31544.Burgener,Ietal;AntibodiesspecificforhumanormurineToll-likereceptorsdetectcanineleukocytesbyflowcytometry.VetImmunolImmunopathol2008,124;1845.Jorgenson,Retal;HumanendometrialepithelialcellscyclicallyexpressToll-likereceptor3(TLR3)andexhibitTLR3-dependentresponsestodsRNA.HumanImmunology2005;66:469StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.DiseaseInfectiousdiseases

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