Description:JAM-A,Human,mAbM.Ab.F11,functionalstudyantibody,lowendotoxinThemonoclonalantibodyM.Ab.F11recognizesjunctionaladhesionmolecule-A(JAM-A)alsoknownasthehumanplateletF11-Receptor(F11R)andJAM-1isacelladhesionmolecule(CAM).JAM-Aisamemberoftheimmunoglobulinsuperfamilyfoundonthesurfaceofhumanplateletsandatintercellularjunctionsofendothelialcellsandepithelialcells.JAM-AbelongstogetherwithJAM-C(JAM-2)andJAM-B(VE-JAMorJAM-3)toafamilyofadhesionproteinswithaV-C2immunoglobulindomainorganization.JAMplaysanimportantroleintightjunctionswhereitisinvolvedincell-to-celladhesionthroughhomophilicinteraction.ItcodistributeswithothertightjunctioncomponentsasZO-1,7H6antigen,cingulinandoccludin.JAM-Aplaysaroleinplateletaggregation,secretion,adhesion,spreADIng.IntheplateletF11R/JAM-Aisamembraneproteininvolvedin2distinctprocessesinitiatedontheplateletsurface.Antibody-inducedplateletaggregationandsecretionbothdependentonFcgammaRIIandGPIIb/IIIaintegrin,aprocessthatmaybeinvolvedinpathophysiologicalprocessesassociatedwithcertainthrombocytopenias.AntibodymediatedplateletadhesionindependentfromFcgammaRIIorfibrinogenreceptorandthatappearstoplayaroleinphysiologicalprocessesassociatedwithplateletadhesionandaggregation.AphysiologicalrolefortheF11R/JAM-Aproteinwasdemonstratedbyitsphosphorylationafterthestimulationofplateletsbythrombinandcollagen.ApathophysiologicalrolefortheF11R/JAM-AwasrevealedbydemonstratingthepresenceofF11R/JAM-Aantibodiesinpatientswiththrombocytopenia.AdhesionofplateletsthroughtheF11Rresultedineventscharacteristicoftheactionofcelladhesionmolecules(CAMs).RecentdatasuggestsaroleforF11R/JAM-Aintheadhesionofplateletstocytokine-inflamedendothelialcellsandthusinthrombosisandatherosclerosisinducedinnon-denudedbloodvesselsbyinflammatoryprocesses.Specifications:CatalognumberHM2099-FSProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mg(100µg/ml)0.2µmfilteredsterileantibodysolutioninPBS(exactconcentrationisindicatedonthelabel)ApplicationFlowcytometry,Functionalstudies,Immunofluorescence,Immunoprecipitation,WesternblotUseForimmunofluorescence,flowcytometryandWesternblottingdilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.IsotypeMouseIgG1SpeciesHumanReferences1.Kornecki,Eetal;Activationofhumanplateletsbyastimulatorymonoclonalantibody.JBiolChem1990,265:100422.WalkowiskBetal;Preferreduseofprimaryradiolabeledanti-plateletmonoclonalantibodies.Comparisonofimmunoblottingmethodsfortheanalysisoffunctionaldomainsonhumanplatelets.ThrombRes1992,68:3233.Naik,Uetal;Mechanismsofplateletactivationbyastimulatoryantibody:cross-linkingofanovelplateletreceptorformonoclonalantibodyF11withtheFcgammaRIIreceptor.BiochemJ1995,310:1554.Wang,Fetal;Stimulatoryantibody-inducedactivationandselectivetranslocationofproteinkinaseCisoenzymesinhumanplatelets.BiochemJ1995,311:4015.Sobocka,Metal;CloningofthehumanplateletF11receptor:acelladhesionmoleculememberoftheimmunoglobulinsuperfamilyinvolvedinplateletaggregation.Blood2000,95:26006.BABInska,Aetal;TworegionsofthehumanplateletF11-receptor(F11R)arecriticalforplateletaggregation,potentiationandadhesion.ThrombHaemost2002,87:712StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseCardiologyandmetabolism,Tumorimmunology