Description:CD36,Human,mAbFA6-152,functionalstudyantibody,lowendotoxinMonoclonalantibodyFA6-152recognizeshumanCD36(88-kDa),acellsurfaceclassBscavengerreceptor,alsoknownasthrombospondinreceptorCD36isaheavilyN-glycosylatedtransmembraneproteinof~88kDawithtwoshortintracellulardomainsandalargeextracellulardomain.Theproteinissensitiveforneuroaminidase,resultinginashiftfrom88to85kDa.CD36isexpressedonplatelets,maturemonocytesandmacrophages,microvascularendothelialcells,mammaryendothelialcells,duringstagesoferythroidcelldevelopmentandonsomemacrophagederiveddendriticcells.Theantibodyrecognizesadultandfetalmonocytes,plateletsandreticulocytes,butdoesn’tstainlymphocytesandgranulocytes.Reactivityhasalsobeenfoundinsmallintestine,kidney,liverandthyroid.CD36expressionisprimarilycontrolledbythetranscriptionheterodimerPPARg-RXR(peroxisomeproliferator-activatedreceptor-g-retinoid-X-receptor).CD36ispreferentiallyfoundwithinlipidrafts,whichfacilitatesitsassociationwithreceptors,signalingandadaptormolecules.Itisareceptorandtransporterofoxidizedlipidsandlongchainfattyacids.CD36hasbeenimplicatedinmanyBIOLOGicalprocessesincludingangiogenesis,phagocytosis,inflammation,andlipidandglucosemetabolism.Severalinvivomodelssupporttheroleofthethrombospondin/CD36systeminangiogenesisandtumorgrowth.AnimportantroleforCD36hasbeenfoundinMalariaasmajorreceptorforP.falciparum-infectedredbloodcells.CD36isassociatedwithSrc-familykinasesandwiththeintegrinsα3β1andα6β1.Recently,CD36hasbeenidentifiedasaproteinthatisrequiredfortolllikereceptor(TLR2)recognitionofdi-acylatedbacteriallipopeptidesandlipoteichoicacid4.FurThermore,CD36hasbeenshowntofunctionasphagocyticreceptorforapoptoticcells.ManydifferentligandshavebeenreportedtointeractwithCD36,suggestingthatCD36couldrecognizeastructure-baseddomainratherthanspecificcontactresidues.MonoclonalantibodyFA6-152blocksthebiologicalactivityofCD36byblockingcollagen/thrombospondinbinding.Theantibodyagglutinatesfetalbutnotadulterythrocytes.Specifications:CatalognumberHM2122-FSProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay.ApplicationFlowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,ImmunoprecipitationApplicationNotesF:Tissueembeddedintissue-tek(forinstanceaortictissue)followedbyfreezinginliquidnitrogen;7-8 µmsections;air-dried;aceton-fixed;10%NGSasblock(Ref4).FC:AntibodyFA6-152stainstheextracellulardomainofCD36.Unfixedcells;2 µgper100.000cells.Positiveongranulocytes(Ref1).FS:Plateletaggregationandsecretionwasinducedby;1 µg/mlantibody(Ref2).IA:10 µg/mlantibodyascoatdilutedinTris-bufferedsaline;100 µm;l/well;o/natRT(Ref3).IF:unfixedcellswereincubatedfor30minutesat4 °CfollowedbyasecondaryFITCpolyclonalantibody;one-minutemethanolfixationbeforeanalysis(Ref1).IP:88kDasialoglycoproteininplatelets;85and88kDainHELcells.10 µgantibody/200 µgproteinUseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Immunogen20-Weeks-oldfetalerythrocytesIsotypeMouseIgG1SpeciesHumanAliasGPIV,Fattyacidtranslocase(FAT),plateletglycoprotein4,PASIV,Plateletcollagenreceptor,thrombospondinreceptorGenename:CD36PositiveControlTHP-1cellsReferences1.EdelmanPetal;AmonoclonalantibodyagainstanerythrocyteontogenicantigenidentifiesfetalandadulterythroidProgenitors.Blood1986,67:56.2.KiefferNetal;Developmentregulatedexpressionofa78kDaerythroblastmembraneglycoproteinimmunologicallyrelatedtotheplateletthrombospondinreceptor.BiochemJ1989,262:8353.ThibertVetal;QuantitationofplateletglycoproteinIV(CD36)inhealthysubjectsandinpatientswithessentialthrombocythemiausinganimmunocaptureassay.ThrombHaemost1992,68:6004.Nakata,Aetal;CD36,anovelreceptorforoxidizedlow-densitylipoproteins,ishighlyexpressedonlidip-ladenmacrophagesinhumanatheroscleroticaorta.ArteriosclerThrombVascBiol1999,19:13335.Ehara,Setal;Pathophysiologicalroleofoxidezedlow-densitylipoproteininplaqueinstABIlityincoronaryarterydiseases.JDiabComplic2002,16:606.Leonarduzzi,Getal;Oxidationasacrucialreactionforcholesteroltoinducetissuedegeneration:CD36overexpressioninhumanpromonocyticcellstreatedwithabiologicallyrelevantoxysterolmixture,AgingCell2008,7:3757.Leonarduzzi,Getal;Molecularsignalingoperatedbyadiet-compatIBLemixtureofoxysterolsinup-regulatingCD36receptorinCD68positivecells,Mol.Nutr.FoodRes.2010,54:S318.Finn,Aetal;HemoglobinDirectsMacrophageDifferentiationandPreventsFoamCellFormationinHumanAtheroscleroticPlaques,JAmCollCardiol.2012,59:1669.Gamba,P.etal.Interactionbetween24-hydroxycholesterol,oxidativestress,andamyloid-βinamplifyingneuronaldamageinAlzheimer’sdisease:threepartnersincrime.AgingCell2011,10:40310.Grange,P.A.etalProductionofSuperoxideAnionsbyKeratinocytesInitiatesP.acnes-InducedInflammationoftheSkin.PlosPathogens2009,5:100052711.Barth,Hetal.ScavengerReceptorClassBIsRequiredforHepatitisCVirusUptakeandCross-PresentationbyHumanDendriticCells.JofVirol.2008,82:346612.Nilsen,Netal.CellulartraffickingoflipoteichoicacidandToll-likereceptor2inrelationtosignaling;roleofCD14andCD36.JournalofLeukocyteBiology2008,84:280StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforatleastoneyear.Theexactexpirydateisindicatedonthelabel.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.DiseaseCardiologyandmetabolism,Infectiousdiseases