首页 > Hycult Biotech > Hycult Biotech/C1q, Mouse, mAb JL-1, functional study antibody, low endotoxin/HM1096-FS/0.5 mg

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Hycult Biotech/C1q, Mouse, mAb JL-1, functional study antibody, low endotoxin/HM1096-FS/0.5 mg

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HM1096-FS

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Description:C1q,Mouse,mAbJL-1,functionalstudyantibody,lowendotoxinThemonoclonalantibodyJL-1recognizesthecollagen-likeregion(CLR)ofmouseC1q,a459kDamoleculeconsistingofthreeindividualpolypeptidechains.TheantibodyhasbeengeneratedbyimmunizationofC1q-/-C57BL/6micewithpurifiedmouseC1q.C1qformstogetherwithC1randC1stheC1macromolecule,thefirstcomponentoftheclassicalcomplementpathway.InteractionofimmunecomplexeswithC1qinducesaconformationalchangewithintheC1complex,whichresultsinactivationoftheclassicalpathway.C1qfunctionsasrecognitionunitbybindingtotheheavychainofIgGorIgM(FcgammaandFcmicro)providedthattheimmunoglobulinsareboundtotheirantigen.FurThermore,C1qcanalsorecognizemolecularpatternsassociatedwithpathogensanditcanbindtoapoptoticblebs,whereitactivatestheclassicalcomplementpathwayandmediatesphagocytosis.Assuch,C1qpromotestheclearanceofapoptoticcellsandsubsequentexposureofautoantigens,therebypreventingstimulationoftheimmunesystem.C1qispredominantlyproducedbymacrophagesbutalsobyfolliculardendriticcells,interdigitatingcellsandcellsofthemonocyte-macrophagelineage.C1qdeficiencyhasaprofoundeffectonhostdefenceandclearanceofimmunecomplexes.AbsenceofC1qmaycauseautoimmunitybyimpairmentoftheclearanceofapoptoticcells.InheritedC1qdeficiencyisalsoassociatedwiththedevelopmentofsystemiclupuserythematosus(SLE).ThemonoclonalantibodyJL-1isreactivewiththecollagen-likeregion(CLR)only,whichisthesameregiontowhichautoantibodiesinmiceandhumansarebinding.Anti-C1qautoantibodiesdepositinglomerulitogetherwithC1qbutinduceovertrenaldiseaseonlyinthecontextofglomerularimmunecomplexdisease.Thisprovidesanexplanationwhyanti-C1qantibodiesareespeciallypathogenicinpatientswithSLE.Specifications:CatalognumberHM1096-FSProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mg(100μg/ml)0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).Theendotoxinconcentrationis<24EU/mg,determinedwithHIT302LALAssay.ApplicationFrozensections,Functionalstudies,Immunoassays,Immunofluorescence,WesternblotApplicationNotesIHC-F:AntibodyJL-1wasusedtostaintissuesectionswhichwerefixedinacetone.Aspositivecontrolapolyclonalanti-C1qantibodywasusedandasnegativecontrolanisotypematchedmonoclonalantibody(Ref.1).FS:AntibodyJL-1wasadmiNISTeredtomiceresultingindepletionofcirculatingC1q,glomerulardepositionofC1qandinductionofanti-C1qautoantibodiesinsusceptIBLemice.Asanegativecontrolanisotypematchedmonoclonalantibodywasused(Ref.1).UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenPurifiedmouseC1qIsotypeMouseIgG2bSpeciesMouseCrossreactivityHuman-Yes,Rat-YesAliasComplementcomponentC1q,ComplementC1qsubcomponentsubunitAPositiveControlSpleenandkidneytissueofwild-typemice(Ref.1)NegativeControlSpleenandkidneytissueofC1q-/-mice(Ref.1)References1.Trouw,Letal;Anti-C1qautoantibodiesdepositinglomerulibutareonlypathogenicincombinationwithglomerularC1q-containingimmunecomplexes.JClinInvest2004,114:6792.Li,Metal;DevelopmentofahumanizedC1qAchainknock-inmouse:assessmentofantibodyindependentbeta-amyloidinducedcomplementactivation.MolImmunol2008,45:32443.Erlich,Petal;ComplementproteinC1qformsacomplexwithcytotoxicprionproteinoligomers.JBiolChem2010,285:19267StorageandstABIlityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct.DiseaseNephrology,Neurologicaldisorders

荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。