首页 > Hycult Biotech > Hycult Biotech/E-selectin, Human, mAb ENA1, immuno assay antibody/HM4001-IA/0.5 mg

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Hycult Biotech/E-selectin, Human, mAb ENA1, immuno assay antibody/HM4001-IA/0.5 mg

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HM4001-IA

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Description:E-selectin,Human,mAbENA1,immunoassayantibodyENA1reactswithE-selectinCD62-E,previousdesignatedtheEndothelialLeucocyteAdhesionMolecule-1(ELAM-1).TheantibodyreactswithhumanendothelialcellsactivatedwithTNF-alpha,IL-1orendotoxin.TheantibodywasfoundtoreactalsowithcellstransfectedwiththeE-selectingene.Theantibodyinhibitstheadhesionofgranulocytesbothneutrophilicandeosinophilic.Specifications:CatalognumberHM4001-IAProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredantibodysolutioninPBS(exactconcentrationisindicatedonthelabel).ApplicationFrozensections,Functionalstudies,Immunoassays,ImmunoprecipitationUseForimmunohistologydilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:10.Invitroculturedcellscanbefixedwith1%paraformaldehydeandkeptinPBSplusazidebeforestaining.Tissuesectionsareadvisedtobefixedfor10mininpureacetoneandfollowedbyincubationfor10mininchloroform.Incubationwithapretesteddilutionoftheantibodyisadvisedtobefollowedbyabiotinconjugatedanti-murineIgandafurtherincubationwithanenzyme(alkalinephosphatase)conjugatedstreptavidin.Forselectionofthemostusefuldilutioninagivensituationateststainingwithcellsortissueknowntoexpresstheantigenshouldbeperformed.Tothisendeitherculturedendothelialcellsorasmallfreshskinbiopsycanbeincubatedfor4hourswithTNF-alpha(1ng/ml),IL-1(100U/ml)orLPS(1µg/ml)intissueculturemediumat37°C.AsnegativecontrolitisadvisedtouseacontrolmurineIgGantibody.IsotypeMouseIgG1SpeciesHumanCrossreactivityBaboon-Yes,Chimpanzee-Yes,Cynomolgusmonkey-Yes,Rhesusmonkey-YesReferences1.Leeuwenberg,JFMetal;Inductionofanactivationantigenonhumanendothelialcellsinvitro.EurJImmunol1989,19:7152.Leeuwenberg,JFMetal;Adhesionofpolymorphonuclearcellstohumanendothelialcells.Adhesionmoleculedependent,andFc-receptor-mediatedadhesion-molecule-independentmechanisms.ClinandExpImmunol1990,81:4963.Leeuwenberg,JFMetal;FunctionalpolymorphismofIgGFcRII(CD32)onhumanneutrophils.Immunology1990,71:3014.Leeuwenberg,JFMetal;Interferon-gammaregulatestheexpressionofanadhesionmoleculeELAM-1andIL-6productionbyhumanendothelialcellsinvitro.JImmunology1990,145:2110StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseCardiologyandmetabolism,Neurologicaldisorders

荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。