首页 > Hycult Biotech > Hycult Biotech/Nitrotyrosine, mAb HM.11, immuno assay antibody/HM5001-IA/0.5 mg

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Hycult Biotech/Nitrotyrosine, mAb HM.11, immuno assay antibody/HM5001-IA/0.5 mg

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HM5001-IA

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Description:Nitrotyrosine,mAbHM.11,immunoassayantibodyThemonoclonalantibodyHM.11recognizesmodifiedaminoacidnitrotyrosineinalldifferentspecies.NitrotyrosineisformedintissuesinpresenceoftheactivemetaboliteNOandisastableendproductofnitrosylationoftyrosine.Inflammationischaracterizedbyincreasednitricoxide(NO)production.NOreactsrapidlywithsuperoxidetoformperoxynitrite.AtphysiologicalpHandinthepresenceoftransitionmetals,peroxynitriteundergoesheterolyticcleavagetoformhydroxylanionandnitroniumion,thelatterofwhichnitratesproteintyrosineresidues.Thepresenceofnitrotyrosinehasbeendetectedinvariousinflammatoryprocessesincludingatheroscleroticplaques,AmyotrophicLateralSclerosis(ALS)andMultipleSclerosis(MS).Thus,thepresenceofnitrotyrosineonproteinscanbeusedasaMarkerforperoxynitriteformationinvivoandconsequentlyasamarkerofNO-mediatedtissuedamage.ThemonoclonalantibodyHM.11recognizesnitrotyrosine,bothwiththefreeaminoacidaswellaswithproteinscontainingnitrotyrosineSpecifications:CatalognumberHM5001-IAProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).ApplicationFrozensections,Immunoassays,Paraffinsections,WesternblotApplicationNotesF:alsocytospins:acetonefixation10min-20 °C;blockendogenousperoxidaseby0.3%H2O2inPBS(ormethanolforintracellularstaining);blockingwith10%NGSor5%BSAfor30min.useatassaydependentconcentrationP:10%formalinfixation;3%H2O2toblockendogenousperoxidases;CitratebufferpH6.0for1minat100 °Casantigenretrievaltreatment;useatassaydependentconcentration(1:200/400)-(Ref1)W:reducedandno-reducedsamples;blockwith5%BSAorskimmedmilk,useatassaydependentconcentrationUseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenNitratedKLHIsotypeMouseIgG2bSpeciesN/APositiveControlW:mousekidneylysate,mouseopticnerve,retina,spinalcordandbrainlysates,rataortalysateP:humanlungtissueReferences1.TerSteege,Jetal;NitrotyrosineinplasmaofceliacdiseasepatientsasdetectedbyanewsandwichELISA.FreeRADIcBiolMed1998,25:9532.Klausz,Getal;LocalandperipheralcytokineresponseandCagAstatusofHelicobacterpylori-positivepatientswithduodenalulcer.EurCytokineNetw2003,14:1433.Casoni,Fetal;Proteinnitrationinamousemodeloffamilialamyotrophiclateralsclerosis.JBiolChem2005,280:162954.Han,Fetal;Proteinnitrationandpoly-ADP-ribosylationinbrainafterrapidexsanguinationscardiacarrestinaratmodelofemergencypreservationandresuscitation.Resuscitation2008,79:3015.Tsuhako,Hetal;Tempolamelioratesmurineviralencephalomyelitisbypreservingtheblood-brainbarrier,reducingviralload,andlesseninginflammation.FreeradicBiolMed2010,48:7046.Brunelli,Letal.Exploratoryinvestigationonnitro-andphospho-proteomecerebellumchangesinhyperammonemiaandhepaticencephalopathyratmodels.Metabolicbraindisease2012,27:377.Nardo,Getal.AmyotrophicLateralSclerosisMultiproteinBiomarkersinPeripheralBloodMononuclearCells.Plosone2011,6:e255458.Yip,P.K.etal.TheOmega-3FattyAcidEicosapentaenoicAcidAcceleratesDiseaseProgressioninaModelofAmyotrophicLateralSclerosis.PlosOne2013,8:61626StorageandstABIlityProductshouldbestoredat-20 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseAutoimmunity,Cardiologyandmetabolism

荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。