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Hycult Biotech/C3b/iC3b/C3c, Mouse, mAb 2/11, immuno assay antibody/HM1065-IA/0.5 mg
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Description:C3b/iC3b/C3c,Mouse,mAb2/11,immunoassayantibodyThemonocolonalantibody2/11recognizesactivatedfragmentsofthemousecomplementproteinC3.Thecomplementsystemisanimportantpartofthehumoralresponseininnateimmunity,consistingofthreedifferentpathways.Thethirdcomplementcomponent,C3,iscentraltotheclassical,alternativeandlectinpathwaysofcomplementactivation.Activationproductsofthecomplementcascadecontainneo-epitopesthatarenotpresentintheindividualnativecomponents.ThesynthesisofC3istissue-specificandismodulatedinresponsetoavarietyofstimulatoryagents.C3isthemostabundantproteinofthecomplementsystemwithserumproteinlevelsofabout1.3mg/ml.AninheriteddeficiencyofC3predisposesthepersontofrequentassaultsofbacterialinfections.Inulcerativecolitis,andidiopathicchronicinflammatoryboweldisease,thedepositionofC3inthediseasedmucosahasbeenreported.ProteolysisbycertainenzymesresultsinthecleavageofC3intoC3aandC3b.C3bbecomesattachedtoimmunecomplexesandisfurthercleavedintoiC3b,C3c,C3dgandC3f.Themonoclonalantibody2/11isspecificforcleavedC3fragmentsC3b,iC3b,andC3c,andforactivatedC3.Therefore,positivereactivityintissuesisassociatedwithactivationofhecomplementcascadeandC3cleavage.IncaseofanacuteinflammatoryreactionlotsofC3areprocessedintotheproductsrecognizedby2/11andisassuchusefulasMarkerforinflammatoryreaction.Inchronicinflammatoryconditionsminimalreactivitywith2/11mayobserved.InsuchcasesprimarilytheC3dgproductresidesattheplaceofinflammation(C3cbeingcleared)whichisnotrecognizedbyantibody2/11.Thechronicprocessing/activationofC3istakingplaceatalowerlevel,whichwouldreducedetectionoftheC3fragmentsC3b,iC3b,andC3c.NexttodetectionofC3fragmentsC3b,iC3b,andC3c,themonoclonalantibody2/11inhibitsthehemolyticactivityofmousecomplementinadose-dependentmanner.Specifications:CatalognumberHM1065-IAProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmfilteredproteinGpurifiedantibodysolutioninPBSwithaconcentrationofatleast0.5mg/ml(exactconcentrationisindicatedonthelabel).ApplicationFlowcytometry,Frozensections,Functionalstudies,Immunoassays,Immunofluorescence,WesternblotApplicationNotesF:Fixationofsectionsinacetonefor5‘,pretreatmentwith3%hydrogenperoxideinmethanolatRTtoquenchendogenousperoxidases.Asnegativecontrol,sectionsfromC3-deficientmicewereused.(Ref.1)FC:Mousesplenocyteswereincubatedwithautologous,freshlydrawnmouseserumtofixtheC3fragmentsbeforestaining.AsnegativecontrolsplenocytesofC3deficientmicewasused.Antibody2/11stainstheneoantigenicsiteontheC3activationfragments.(Ref.1)FS:Antibody2/11inhibitshemolysisinadose-dependentmanner.(Ref.1)W:Anon-reducedsampletreatmentandSDS-PAGEwasused.Thebandsizeis150kDa(Ref.1).IA:Supernatantofantibody2/11wasincubatedonanti-ratIgGFccoatedplates.Afterincubationwithsample,HRP-conjugatedpolyclonalgoatanti-mouseC3wasusedfordetection.(Ref.1)UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenMouseC3IsotypeRatIgG1SpeciesMousePositiveControlMousesplenocytespreincubatedwithserumfromthesameanimal.NegativeControlC3-deficientmousecells.References1.Mastellos,Detal;NovelmonoclonalantibodiesagainstmouseC3interferingwithcomplementactivation:descriptionoffinespecificityandapplicationstovariousimmunoassays.MolImmunol2004,40:12132.Markiewski,Metal;C3aandC3bactivationproductsofthethirdcomponentofcomplement(C3)arecriticalfornormalliverrecoveryaftertoxicinjury.JImmunol2004,173:7473.Markiewski,Metal;Modulationoftheantitumorimmuneresponsebycomplement.NatImmunol2008,9:1225StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseInfectiousdiseases,Nephrology

荷兰HyCult Biotechnology(HBT)位于荷兰乌登,从1994开始制造检测抗体和蛋白质,主要侧重与病理学诊断有关的全套产品研发,既有纯化的重组蛋白和多肽,也有单抗和多抗,是世界一流的先天免疫领域的研究试剂制造商。

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