首页 > Hycult Biotech > Hycult Biotech/Elastase, Human, mAb 265-3K1, immuno assay antibody/HM2174-IA/0.5 mg

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Hycult Biotech/Elastase, Human, mAb 265-3K1, immuno assay antibody/HM2174-IA/0.5 mg

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HM2174-IA

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Description:Elastase,Human,mAb265-3K1,immunoassayantibodyThemonoclonalantibody265-3K1recognizeshumanleukocyteelastase.Leukocyteelastase,amajorserineproteinaseinman,ispredominantlypresentintheazurophilicgranulesofneutrophilsandmonocytes.Elastasehasabroadrangeofextracellularmatrixsubstratesincludingelastin,proteoglycans,collagenandfibronectin.Theactionofelastaseiscontrolledbyserineproteinaseinhibitors.Elastase,whenreleasedduringinflammation,israpidlyboundbyitstwomaininhibitors,alpha1-PIandalpha2-macroglobulinetoformelastase-inhibitorcomplexes.Inadditionmucosasecretionsmaycontainthelocallysecretedelastaseinhibitorselafin/SKALPandSLPI.Whensecretedatsitesofinflammationelastasecancauseseveretissuedamage.Animportantrolehasbeensuggestedforhumanelastaseinvariousinflammatorydisorders,includingpulmonaryemphysema,sepsis,arthritis,nephritisandcertainskindiseases.ElastaseinducestheproductionofIL-8inhumanbronchialepithelial,aprocesthatoccursinpartthroughTLR4.Specifications:CatalognumberHM2174-IAProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2µmfilteredproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel).ApplicationImmunoassays,WesternblotApplicationNotesIA:theantibodycanbeusedascaptureantibody.Antibody265-3K1wasuniqueinthatthelimitofdetectionasacaptureantibodyincreasedfrom625nM(18.8ng/ml)forfreeHNEto80nMforHNE–AATcomplex,indicatingthatcovalentcomplexformationbetweenHNEandAATincreasesantibodyaffinityorincreasesexposureofrecognisedepitopeinHNEfor265-3K1(Ref.1).FC:cellswerefixedwithADGfixationbufferAfor15minatroomtemperature.Cellswerewashedandincubatedwith10μgofmonoclonalanti-HNEantibodydilutedinADGpermeABIlisationbufferB(Ref.1).IF:tissueswerefixedinparaformaldehyde(PFA)3.7%,embeddedinparaffinandsectionedat6mm.Thesectionsweredeparaffinizedintolueneandhydratedingradedseriesofethanol(Ref.2).UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.IsotypeMouseIgG1SpeciesHumanReferences1.Davies,Petal;Monoclonalanti-neutrophilelastaseantibodycharacterisation:abilitytoblockfunction,detectfreeversusserpin-complexedenzymeandstainintracellulargranules.JImmunol.Meth.2008,336:1752.Delbosc,Setal;PorphyromonasgingivalisParticipatesinPathogenesisofHumanABDominalAorticAneurysmbyNeutrophilActivation.ProofofConceptinRats.PLosONE2011,6:e186793.Novak,Tetal;OralUlcerationinBehçet"sDisease:AnInvestigationofNeutrophilElastaseandItsInhibitors.Thesis2013StorageandstabilityProductshouldbestoredat4°C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseAutoimmunity,Pulmonology

荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。