首页 > Hycult Biotech > Hycult Biotech/H-FABP, Human, mAb 67D3, immuno assay antibody/HM2018-IA/0.5 mg

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Hycult Biotech/H-FABP, Human, mAb 67D3, immuno assay antibody/HM2018-IA/0.5 mg

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HM2018-IA

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Description:H-FABP,Human,mAb67D3,immunoassayantibodyThemonoclonalantibody67D3recognizeshumanheart-typefattyacid-bindingprotein(H-FABP)ofbothnaturalandrecombinantorigin.TheH-FABPproteinisderivedfromthehumanFABP3gene.FABPsaresmallintracellularproteins(~13-14kDa)withahighdegreeoftissuespecificitythatbindlongchainfattyacids.Theyareabundantlypresentinvariouscelltypesandplayanimportantroleintheintracellularutilizationoffattyacids,transportandmetabolism.ThereareatleastninedistincttypesofFABP,eachshowingaspecificpatternoftissueexpression.Duetoitssmallsize,FABPleaksrapidlyoutofischemicallydamagednecroticcellsleADIngtoariseinserumlevels.Ischemicallydamagedtissuesarecharacterizedhistologicallybyabsence(orlowpresence)ofFABPfacilitatingrecognitionofsuchareas.H-FABPislocalizedintheheart,skeletalandsmoothmuscle,mammaryepithelialcells,aorta,distaltubulesofthekidney,lung,brain,placenta,andovary.FurThermore,thisantibodyisusefulforthepurificationofH-FABP.Specifications:CatalognumberHM2018-IAProducttypeMonoclonalantibodiesQuantity0.5mgFormulation0.5mgof0.2μmproteinGpurifiedantibodysolutioninPBS(exactconcentrationisindicatedonthelabel).ApplicationFrozensections,Immunoassays,Immunoprecipitation,WesternblotApplicationNotesIP:Biotinylated67D3wasimmobilizedonstreptavidinbeadsandaddedtoserumtoimmunoprecipitateH-FABP(Ref.3).W:Reducedsampletreatment.Thebandsizeis~15kDa(Ref.2).F:PermeABIlizationwasdoneincoldacetonewith0.5%hydrogenperoxidasefor10min,afterdryingandwashing,antibodieswereincubatedfor30minutes.UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenPurifiedHumanH-FABPIsotypeMouseIgG1SpeciesHumanCrossreactivityMouse-Yes,Rat-YesAliasHeart-typefattyacid-bindingprotein,Fattyacid-bindingprotein3,FABP3,Musclefattyacid-bindingprotein,Mammary-derivedgrowthinhibitorGenename:FABP3,FABP11,MDGIPositiveControlHeartcells,recombinanthumanH-FABPNegativeControlTonsiltissueReferences1.Roos,Wetal;Monoclonalantibodiestohumanheartfattyacid-bindingprotein.JImmunolMeth1995,183:1492.Pelsers,Metal;Brain-andheart-typefattyacid-bindingproteinsinthebrain:tissuedistributionandclinicalutility.ClinChem2004,50:15683.Zhen,Eetal;QuantificationofheartfattyacidbindingproteinasabioMarkerfordrug-inducedcardiacandmusculoskeletalnecrosis.ProteomicsClinAppl2007,1:661StorageandstabilityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultwiththeuseorderivationofthisproduct.DiseaseCardiologyandmetabolism,Neurologicaldisorders

荷兰HyCult Biotechnology(HBT)位于荷兰乌登，从1994开始制造检测抗体和蛋白质，主要侧重与病理学诊断有关的全套产品研发，既有纯化的重组蛋白和多肽，也有单抗和多抗，是世界一流的先天免疫领域的研究试剂制造商。