Description:CD34,Mouse,mAbMEC14.7ThemonoclonalantibodyMEC14.7recognizesmouseCD34,asingle-passtypeImembraneglycophosphoproteinpresentonsmallvesselendothelialcellsandhematopoieticProgenitorcells.TheapparentmolecularmassofCD34isheterogeneous,dependingontheglycosylationstateindifferentcelltypes.Inculturedendotheliomacelllysate,CD34hasamolecularweightof~100kDa,whereasinlunglysatesitis~80kDa.2IsoformsofCD34exist,bothareexpressedonthecellsurface.CD34isanadhesionmoleculeperformingaroleinearlyhematopoiesisbymediatingtheattachmentofstemcellstothebonemarrowextracellularmatrixordirectlytostromalcells.CD34actsasascaffoldfortheattachmentoflineagespecificglycans,allowingstemcellstobindtolectinsexpressedbystromalcellsorothermarrowcomponents.CD34presentscarbohydrateligandstoselectins.CD34iswidelyusedasaMarkertoselectearlyhematopoieticstemandprogenitorcellsinexperimentalandclinicalhematopoiesis.ThemonoclonalantibodyMEC14.7recognizesaneuraminidasesensitiveepitopeonendotheliuminvivo,particularlyonsmallvesselsandneoformedcapillariesanddevelopingvascularstructuresinembryonalstructures.ThemonoclonalantibodyMEC14.7canbeusedforidentificationandcharacterizationofcapillaryendothelialcells.FurThermore,theantibodyisusefulforisolationandcharacterizationofhematopoieticprogenitorcells,particularlyofmyelomonocyticcolonyformingcells.MonoclonalantibodyMEC14.7isalsousefulforimmunopurificationandcellseparationSpecifications:CatalognumberHM1015ProducttypeMonoclonalantibodiesQuantity100µgFormulation1ml(100µg/ml)0.2µmfilteredantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide.ApplicationFlowcytometry,Frozensections,Immunofluorescence,Immunoprecipitation,Paraffinsections,WesternblotApplicationNotesW:bothreduced/non-reduced;70-105kDa,dependingonglycosylationstateandcelltype(Ref1)P:Formalinfixed;boiledtwicefor5minutesincitrate(pH6.0)asantigenretrieval(inhousetested;Ref4,6)or0.03%trypsintreatmentfor10minutesat37 °C(Ref3)FC:micebloodcells;0.1 µgMEC14.7/106cells(Ref5)IP:~100kDaproteininH5Vcells(Ref1)CD34inmouseglioma.StainingofparaffintissuesectionwithantibodyMEC14.7(Cat.#HM1015).Anti-mouseCD34at5 µg/ml(o/n,4 °C)resultedinthespecificstainingofendothelialcells.UseForimmunohistology,flowcytometryandWesternblotting,dilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.Forfunctionalstudies,invitrodilutionshavetobeoptimizedinuser"sexperimentalsetting.ImmunogenMurinetransformedECt-end.1IsotypeRatIgG2aSpeciesMouseAliasHematopoieticprogenitorcellantigenCD34PositiveControlH5VcellsReferences1.Garlanda,Cetal;CharacterizationofMEC14.7,anewmonoclonalantibodyrecognizingmouseCD34,ausefulreagentforidentifyingandcharacterizingbloodvesselsandhematopoieticprecursors.EurJCellBiol1997,73:3682.Dong,Qetal;Ageneralstrategyforisolationofendothelialcellsfrommurinetissues:Characterizationoftwoendothelialcelllinesfromthemurinelungandsubcutaneousspongeimplants.ArteriosclerThrombVascBiol1997,17:15993.Solberg,Hetal;AfunctionaloverlapofplasminogenandMMPsregulatesvascularizationduringplacentaldevelopment.Development2003,130:44394.Almholt,Ketal;Metastasisoftransgenicbreastcancerinplasminogenactivatorinhibitor-1gene-deficientmice.Oncogene2003,22:43895.Sho,Eetal;HemodynamicregulationofCD34+celllocalizationanddifferentiationinexperimentalaneurysms.ArteriosclerThrombVascBiol2004,24:19166.Suganuma,Tetal;FunctionalexpressionoftheangiotensinIItype1receptorinhumanovariancarcinomacellsanditsblockadetherapyresultinginsuppressionoftumorinvasion,angiogenesis,andperitonealdissemination.ClinCancerRes2005,11:2686StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandfederalrulesintheuseofthisproduct.HycultBiotechisnotresponsIBLeforanypatentinfringementsthatmightresultfromtheuseorderivationofthisproduct.DiseaseTumorimmunology