Description:Mannosereceptor,Human,mAb15-2,biotinylatedThemonoclonalantibody15-2recognizesthemannosereceptor(MR),alsoknownasCD206,amemberofthevertebrateC-typelectinfamily.Themannosereceptor,isapatternrecognitionreceptorthatisinvolvedinbothinnateandadaptiveimmunity.The175kDasingle-passtypeItransmembranereceptorconsistsof5domains:anamino-terminalcysteine-richregion,afibronectintypeIIrepeat,aseriesofeighttandemlectin-likecarbohydraterecognitiondomains(responsIBLefortherecognitionofmannoseandfucose),atransmembranedomain,andanintracellularcarboxy-terminaltail.Thestructureissharedbythefamilyofmultilectinmannosereceptors:thephospholipaseA2-receptor,DEC205andthenovelC-typelectinreceptor(mannosereceptorX).TheMRbindshigh-mannosestructureson-awiderangeofgrampositiveandgramnegativebacteria,yeasts,parasitesandmycobacteria.TheMRhasalsobeenshowntobindandinternalizetissue-typeplasminogenactivator.MR"sarepresentonmonocytesanddendriticcells(DC)andarepresumedtoplayaroleininnateandadaptiveimmunity,thelatterviaprocessingbyDC.TheexpressionofMRasobservedinimmunohistologyispresentontissuemacrophages,dendriticcells,asubpopulationofendothelialcells,Kupffercellsandspermcells.TheexpressionofMRonmonocytesincreasesduringcultureandcanbeenhancedbycytokinesasIFN-gamma.LabelingofMRexpressingmonocytes/macrophagesincreaseswithprolongedincubationtimeprobablyduetointernalizationoftheMR-antibody-complex.Themonoclonalantibody15-2preventsbindingofglycoproteinsincludingt-PAtoMR.DetectionoftheMRwithanti-MRmonoclonalantibody15-2cansubstitutestainingformannosecontainingprobesaslabeledmannosylatedBSA,atechniquewhichismorecumbersomeandlessspecific.Specifications:CatalognumberHM2057ProducttypeMonoclonalantibodiesQuantity50µgFormulation0.5ml(100μg/ml)0.2μmfilteredproteinGpurifiedbiotinylatedantibodysolutioninPBS,containing0.1%bovineserumalbuminand0.02%sodiumazide.ConjugateBiotinApplicationFlowcytometry,Frozensections,Functionalstudies,WesternblotApplicationNotesF:Tissuesectionswerefixedinacetone/chloroform.Sectionswerepreincubatedwithnormalrabbitserum(Ref.3).FC:Antibody15-2stainstheextracellulardomainofMR.W:Anon-reducedsampletreatmentand6%SDS-Pagewasused.Thebandsizeis175kDa(Ref.1).FS:Antibody15-2functionsasaninhibitorofbinding.Theantibodywasfunctionallytestedbypreincubationofimmobilizedreceptorwithantibody.Thispreventedbindingoft-PAtoimmobilizedreceptor.(Ref.1).UseDilutionstobeuseddependondetectionsystemapplied.Itisrecommendedthatuserstestthereagentanddeterminetheirownoptimaldilutions.Thetypicalstartingworkingdilutionis1:50.ImmunogenPurifiedhumanmannosereceptorfromhumanplacentaltissueIsotypeMouseIgG1SpeciesHumanAliasMachrophageMannoseReceptor1,MRC1,CLEC13D,MMR,C-typelectindomainfamily13memberDGenename:MRC1PositiveControlMacrophages(incoronaryatheroscleroticplaque),placentatissue,Hofbauercells(fetalmacrophages),livertissueNegativeControlLymphocytes,MonocytesReferences1.Barret-Berghoeff,Metal;Monoclonalantibodiesagainstthehumanmannosereceptorthatinhibitthebindingoftissue-typeplasminogenactivator.ThrombHaemostas1997,77:7182.Noorman,Fetal;MonoclonalantibodiesagainstthehumanmannosereceptorasaspecificMarkerinflowcytometryandimmunohistochemistryformacrophages.JLeukocyteBiol1997,61:633.Noorman,Fetal;Clustermannosidescaninhibitmannosereceptor-mediatedtissue-typeplasminogenactivatordegradationbybothratandhumancells.Hepatology1997,26:13034.Tailleux,Letal;DC-SIGNisthemajormycobacteriumtuberculosisreceptoronhumandendriticcells.JExpMed2003,197:1215.Pressice,Petal;Keyholelimpethemocyanininducestheactivationandmaturationofhumandendriticcellsthroughtheinvolvementofmannosereceptor.MolImmunol2008,45:11366.Tahara,Netal;2-deoxy-2-[(18)F]fluoro-d-mannosepositronemissiontomographyimaginginatherosclerosis.NatMed2014,20:215StorageandstABIlityProductshouldbestoredat4 °C.Underrecommendedstorageconditions,productisstableforoneyear.PrecautionsForresearchuseonly.Notforuseinoronhumansoranimalsorfordiagnostics.Itistheresponsibilityoftheusertocomplywithalllocal/stateandFederalrulesintheuseofthisproduct.HycultBiotechisnotresponsibleforanypatentinfringementsthatmightresultwiththeuseoforderivationofthisproduct.DiseaseCardiologyandmetabolism,Infectiousdiseases