首页 > ACROBiosystems > ACROBiosystems/Human BTN3A1 / CD277 Protein, Fc Tag/1mg/BT1

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ACROBiosystems/Human BTN3A1 / CD277 Protein, Fc Tag/1mg/BT1

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SynonymBTN3A1,CD277,BTF5SourceHuman BTN3A1, Fc Tag (BT1-H5255) is expressed from human 293 cells (HEK293). It contains AA Gln 30 - Gly 254 (Accession # AAI21801).Predicted N-terminus: Gln 30Request for sequenceMolecular CharacterizationThis protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 50.8 kDa. As a result of glycosylation, the protein migrates as 58-65 kDa under reducing (R) condition, and 120 kDa under non-reducing (NR) condition (SDS-PAGE).EndotoxinLess than 1.0 EU per μg by the LAL method.Purity>95% as determined by SDS-PAGE.FormulationLyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.Contact us for customized product form or formulation.ReconstitutionPlease see Certificate of Analysis for specific instructions.For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.StorageFor long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. SDS-PAGEHuman BTN3A1, Fc Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%. BackgroundButyrophilin subfamily 3 member A1 (BTN3A1) is also known as CD277 and BTF5, which belongs to the immunoglobulin superfamily and contains one B30.2/SPRY domain and two Ig-like V-type (immunoglobulin-like) domains. BTN3A1 plays a role in T-cell activation and in the adaptive immune response. Also, BTN3A1 regulates the proliferation of activated T-cells and the release of cytokines and IFNG by activated T-cells. Furthermore, BTN3A1 mediates the response of T-cells toward infected and transformed cells that are characterized by high levels of phosphorylated metabolites, such as isopentenyl pyrophosphate. ReferencesPlease contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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