首页 > ACROBiosystems > ACROBiosystems/Human RNF43 Protein, Fc Tag (MALS verified)/100ug/RN3

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ACROBiosystems/Human RNF43 Protein, Fc Tag (MALS verified)/100ug/RN3

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SynonymRING finger protein 43,RING-type E3 ubiquitin transferase RNF43SourceHuman RNF43, Fc Tag (RN3-H5256) is expressed from human 293 cells (HEK293). It contains AA Gln 44 - Tyr 197 (Accession # Q68DV7-1).Predicted N-terminus: Gln 44Request for sequenceMolecular CharacterizationThis protein carries a human IgG1 Fc tag at the C-terminus.The protein has a calculated MW of 43.5 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.EndotoxinLess than 1.0 EU per μg by the LAL method.Purity>95% as determined by SDS-PAGE.FormulationPlease contact us for detailed information.Contact us for customized product form or formulation.ReconstitutionPlease see Certificate of Analysis for specific instructions.For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.StorageFor long term storage, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. SDS-PAGEHuman RNF43, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.SEC-MALSThe purity of Human RNF43, Fc Tag (Cat. No. RN3-H5256) was more than 85% and the molecular weight of this protein is around 100-115 kDa verified by SEC-MALS.ReportBioactivity-ELISAImmobilized Human R-Spondin 1, His Tag (Cat. No. RS1-H4221) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 2-20 ng/mL (QC tested).ProtocolImmobilized Human R-Spondin 3 (22-146), His Tag (Cat. No. RS6-H5222) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.3-2 ng/mL (Routinely tested).ProtocolImmobilized Human R-Spondin 1 (21-146), His Tag (Cat. No. RS6-H4220) at 5 μg/mL (100 μL/well) can bind Human RNF43, Fc Tag (Cat. No. RN3-H5256) with a linear range of 0.01-0.156 μg/mL (Routinely tested).Protocol BackgroundE3 ubiquitin-protein ligase that acts as a negative regulator of the Wnt signaling pathway by mediating the ubiquitination, endocytosis and subsequent degradation of Wnt receptor complex components Frizzled. Acts on both canonical and non-canonical Wnt signaling pathway. Along with RSPO2 and ZNRF3, constitutes a master switch that governs limb specification (By similarity) ReferencesPlease contact us via TechSupport@acrobiosystems.com if you have any question on this product.

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