产品说明
Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.Product HighlightsReproducible – consistent results between technical replicates for increased confidence in resultsSpecific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliabilitySensitive – reliable quantification of low abundance targets and scarce samplesRobust – reliable, accurate detection of DNA and RNA targets from a broad range of sample typesFast – delivers reproducible, accurate assay results in as little as 30 minutesProduct DescriptionThe SensiFAST™ SYBR No-ROX Kit has been developed for fast, highly accurate real-time PCR and has been validated on all commonly-used real-time instruments that do not require the passive reference dye ROX.A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR No-ROX Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.The SensiFAST SYBR®No-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.Request InfoApplicationsGene expression analysisDNA / RNA target detectionmiRNA profiling / quantificationCopy number variation (CNV) analysis Fig. 1 SensiFAST SYBR using fast cycling conditionsThe rps18 gene was amplified using SensiFAST SYBR No-ROX used a 10 fold serial dilution of cDNA over four orders of magnitude. The results illustrate that SensiFAST SYBR No-ROX is a fast (early Ct), highly reproducible and sensitive kit, with do detectable primer-dimers.SensiFAST Products Customer Review SensiFAST SYBR No-ROX One-Step Kit Customer ReviewReal-Time PCR Selection ChartOne-step Vs. Two-step real-time RT PCR A discussion of the pros and cons of each detection strategy.
Bioline成立于1992年，总部设在英国伦敦；随着时间推移，信息化的高速发展，Bioline目前已经成国际化公司，其分部设在美国，德国，法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司，是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量，定制和OEM的dNTP，我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力，研发和制造的主要活动领域包括：实时定量PCR试剂盒，DNA聚合酶，逆转录酶，克隆试剂，核酸分离，超纯核苷酸合成，DNA与RNA分子量Marker，感受态细胞，分子生物学试剂，蛋白的表达及纯化。2010年，Meridian Life Science收购了 Bioline 公司。
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