Outstanding assay reproducibility, sensitivity and robustness from both DNA and RNA templates, under fast thermal cycling conditions.Product HighlightsReproducible – consistent results between technical replicates for increased confidence in resultsSpecific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliabilitySensitive – reliable quantification of low abundance targets and scarce samplesRobust – reliable, accurate detection of DNA and RNA targets from a broad range of sample typesFast – delivers reproducible, accurate assay results in as little as 30 minutesProduct DescriptionThe SensiFAST™ SYBR & Fluorescein Kit has been developed for fast, highly accurate real-time PCR and has been validated on real-time PCR platforms that require the passive reference dye fluorescein for collection of dynamic well factors.A combination of the latest advances in buffer chemistry and PCR enhancers ensures that the SensiFAST SYBR & Fluorescein Kit produces reliable assay results under fast thermal cycling conditions. An antibody-mediated hot-start DNA polymerase system promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range.The SensiFAST SYBR & Fluorescein Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.Request InfoApplicationsGene expression analysisDNA / RNA target detectionmiRNA profiling / quantificationCopy number variation (CNV) analysis Fig. 1 SensiFAST SYBR using fast cycling conditionsA fragment of γ-actin was amplified using SensiFAST SYBR & Fluorescein (red) and the results were compared with amplification using a kit from Supplier I (blue). The process using a 10 fold serial dilution of human genomic DNA over four orders of magnitude. The results illustrate that SensiFAST SYBR & Fluorescein is as faster (earlier Ct) and more efficient than Supplier I. Fig. 2 Sensitivity of SensiFAST SYBR & FluoresceinA fragment of ubiquitin gene was amplified using SensiFAST SYBR & Fluorescein (red) and the results were compared with amplifications using a Kit from supplier Q (blue). The process used a 10 fold serial dilution of human RNA (in triplicate) over five orders of magnitude. The results illustrate that a) SensiFAST SYBR & Fluorescein was faster (earlier Ct) and more sensitive than supplier Q and b) there was far less primer-dimer formation.Highly efficient under fast conditions. SensiFAST Products Customer Review SensiFAST SYBR No-ROX One-Step Kit Customer ReviewReal-Time PCR Selection ChartOne-step Vs. Two-step real-time RT PCR A discussion of the pros and cons of each detection strategy.