Sensitive, reproducible detection of even the most challenging RNA targets, under fast thermal cycling conditions.Product HighlightsSensitive – optimized buffer formulation delivers reliable quantification from even very low copy number RNA targetsReproducible – consistent results between technical replicates for increased confidence in resultsRobust – reliable detection of RNA targets from a broad range of sample typesFast – delivers reproducible, accurate assay results in as little as 40 minutesEfficient – excellent performance in multiplex assaysSpecific – antibody-mediated hot-start DNA polymerase minimizes non-specific amplification for improved assay sensitivity and reliabilityProduct DescriptionThe SensiFAST Probe Hi-ROX One-Step Kit has been optimized for fast, efficient, unbiased cDNA synthesis and subsequent highly-sensitive, reproducible real-time PCR detection in a single tube. SensiFAST Probe One-Step has been optimized to deliver excellent results in both singleplex and multiplex assays.An antibody-mediated hot-start DNA polymerase promotes rapid activation and supports highly-specific amplification, which in turn improves assay sensitivity and dynamic range. A combination of the latest advances in buffer chemistry and PCR enhancers confer superior assay performance under fast thermal cycling conditions. The inclusion of separate RiboSafe Inhibitor ensures accuracy by protecting RNA targets from RNase degradation.The SensiFAST™ Probe Hi-ROX One-Step Kit has been validated on all commonly-used real-time instruments that require a high concentration of the passive reference dye ROX. SensiFAST Probe One-Step has been formulated for use with dual-labelled probes, including TaqMan®, Scorpions® and molecular beacon probes.Request InfoApplicationsGene expression analysisPathogen detectionRNA viral pathogen detectionGenetic profilingmiRNA profiling / quantificationGenetically modified organisms (GMO) characterization Mouse β-actin was amplified in triplicate using gene-specific primers and TaqMan probe according to each manufacturer"s protocol, from 10-fold serial dilution of RNA, using either SensiFAST Probe One-Step Kit (red) or similar kits from Suppliers A (green) and Q (blue). The SensiFAST Probe One-Step Kit gives the lowest Ct values while maintaining reproducibility of technical replicates. A fragment of the mouse β-actin gene was amplified in triplicate from a 10-fold serial dilution of mouse 3T3 total RNA using SensiFAST Probe One-Step Kit, gene-specific primers and a TaqMan probe, under fast thermal cycling conditions. The amplification plot shows excellent assay reproducibility. A 100-fold dilution of human cDNA was targeted with 4 different gene-specific assays, in either singleplex reactions (dark coloured line) or quadruplex assays (light coloured line), using the SensiFAST Probe One-Step Kit and similar kits from Suppliers Q and T. The results demonstrate that SensiFAST Probe One-Step Kit is very efficient in multiplexing, as reactions containing single and four probes give the same Ct value, unlike the kits from Suppliers Q and T.Unparalleled performance under fast conditions. Introduction to SensiFAST Overview, features and benefits of the SensiFAST product familyReal-Time PCR Selection ChartOne-step Vs. Two-step real-time RT PCR A discussion of the pros and cons of each detection strategy.