产品说明
DescriptionA robust, efficient, proofreading enzyme that gives increased fidelity in high-yield PCR, for use in all routine cloning applications.Product HighlightsEfficient – highly productive target amplification and removal of 3’ A overhangsAccurate – possesses 3’ - 5’ proofreading exonuclease activity that delivers an error rate of 3.0 x 10-6 for increased PCR fidelity versus Taq DNA polymeraseSensitive – high-yield amplification from limiting amounts of human, animal and plant template DNARobust – developed for reliable amplification of even the most challenging targets, including genomic DNA and GC-rich targetsFlexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samplesConvenient – advanced buffering system minimizes the requirements for PCR optimization, thereby reducing time-to-results and eliminating the cost of unnecessary repeatsProduct DescriptionACCUZYME™ is a proprietary proofreading enzyme that offers increased-fidelity and high PCR yield, even in demanding applications. ACCUZYME has an error-rate of 3.0 x 10-6 and results in blunt-ended amplicons up to 5 kb in length, making it ideal for use in cloning and site-directed mutagenesis.ACCUZYME is supplied with a buffering system that provides ideal conditions for most PCR assays. Consequently, the cost and effort typically associated with optimizing assay performance is often eliminated. In circumstances where further optimization is required to improve PCR specificity and/or yield, ACCUZYME includes an additional vial of MgCl2.Request InfoApplicationsHigh-fidelity PCRRoutine cloning applications requiring increased PCR yieldBlunt-end cloningSite-directed mutagenesis An 800 bp fragment was amplified from 500 ng, 50 ng, 5 ng, 0.5 ng, 50 pg and 5 pg (lanes 1-6 respectively) of human genomic DNA using ACCUZYME. HyperLadder 1kb (M).The result illustrates that ACCUZYME gives high product yield even from limiting amounts of template. ACCUZYME™ Mix Customer ReviewPCR Enzyme Guide Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research PCR Selection Chart Select the best reagent for your research
Bioline成立于1992年，总部设在英国伦敦；随着时间推移，信息化的高速发展，Bioline目前已经成国际化公司，其分部设在美国，德国，法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司，是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量，定制和OEM的dNTP，我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力，研发和制造的主要活动领域包括：实时定量PCR试剂盒，DNA聚合酶，逆转录酶，克隆试剂，核酸分离，超纯核苷酸合成，DNA与RNA分子量Marker，感受态细胞，分子生物学试剂，蛋白的表达及纯化。2010年，Meridian Life Science收购了 Bioline 公司。
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