产品说明
DescriptionThe ready-to-use molecular grade 100 mM solution of dCTP as lithium salts in purified water.Product HighlightsHigh quality - >99% purity determined by HPLC, ideal for use in PCR reactionsLong shelf-life - no need for aliquotingConvenient format – ready-to-use concentrationEnzyme free - DNase, RNase and Nickase freeFlexible sizes - custom, bulk and OEM sizes availableProduct DescriptionHighly quality dNTPs are a vital requirement for successful PCR, as the presence of contaminating impurities will result in a decrease in amplification sensitivity and product yield. Bioline ultra-pure dCTP undergo highly stringent purification steps to give a greater than 99% purity and are tested for the absence of DNase, RNase, Protease, Nickase activity. It is then quality controlled in a variety of applications, ensuring that it is highly suitable for the most sensitive techniques.Bioline dCTP is supplied as lithium salts in purified water, as lithium salts have greater resistance to repeated freezing and thawing cycles than sodium salts and remain sterile due to the bacteriostatic activity of lithium towards various microorganisms, giving greater reliability and an extended shelf life.As an ISO 13485 certified primary manufacturer of nucleotides, if your requirements for dCTP is beyond the scope of our standard product, Bioline can also offer custom, bulk and OEM services (custom@bioline.com).Request InfoApplicationsDNA amplification (standard, long-range, multiplex and high-fidelityPCR)RT-PCRqPCRGenotypingcDNA synthesisDNA sequencingDNA labelingMutagenesisExpression profilingEpigenetic analysis Fig. 1 Multiple rounds of freeze-thawingTwo different batches of dNTPs were freeze-thawed 5, 10 and 20 times and two-fold serial dilution was used in a PCR reaction. Both batches produced identical PCR products of 2 kb whether freeze-thawed 5 times or freeze-thawed 20 times. Fig. 2 Long-term storageLong-term stability of dNTPs was determined on dNTPs stored at -20oC for one year and two years. A two-fold serial dilution of these dNTPs was used in a PCR reaction to produce identical PCR products regardless of age of the dNTPs.
Bioline成立于1992年,总部设在英国伦敦;随着时间推移,信息化的高速发展,Bioline目前已经成国际化公司,其分部设在美国,德国,法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司,是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量,定制和OEM的dNTP,我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力,研发和制造的主要活动领域包括:实时定量PCR试剂盒,DNA聚合酶,逆转录酶,克隆试剂,核酸分离,超纯核苷酸合成,DNA与RNA分子量Marker,感受态细胞,分子生物学试剂,蛋白的表达及纯化。2010年,Meridian Life Science收购了 Bioline 公司。
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