DescriptionA high-fidelity polymerase mix using aptamer-based hot-start technology, highly suited to amplification of DNA from crude samples, for fast, inexpensive target enrichment, NGS library amplification and cloning applications.Product HighlightsRobust - optimized enzyme/buffer mix promotes reliable amplification of a broad range of targets, including complex DNA extracted from human, animal and plant samplesSpecific - the aptamer-based hot-start remains completely inactive during PCR set-up and after amplification, to prevent non-specific productsOptimized - high yields withminimal optimization, regardless of a template’s GC content, reducing time to results and eliminating the cost of unnecessary repeatsEnhanced accuracy - higher than 130x Taq fidelity, reducing errors for next generation sequencing (NGS) library amplificationProduct DescriptionSimpliFi HS Mix is a combination of the latest advances in buffer chemistry and PCR enhancers and stabilizers, together with an aptamer-mediated hot-start polymerase, dNTPs and MgCl2. It has been designed for highly reproducible, accurate assay results in the presence of inhibitors. The advanced buffer chemistry and enhancers has been developed for fast PCR and is designed for superior sensitivity and specificity, making SimpliFi HS Mix perfect for NGS library amplification.ApplicationsGene expression Viral and bacterial detection Robust PCR High-specificity PCRHigh-fidelity PCR GC/AT-rich PCR Fidelity comparison across commercially available high-fidelity polymerases.The fidelity of the SimpliFi HS Mix was determined using a method designed by Lee D. et al. (Mapping DNA polymerase errors by single-molecule sequencing) using next generation sequencing and compared to other commercially available high-fidelity master mixes, using the same method. The results illustrate the higher fidelity (130x) of SimpliFi HS Mix. GC bias with both high and low GC contentGC bias plot from whole-genome sequencing of a mixture of S. aureus and R. spheroides genomic DNA using SimpliFi HS Mixand a high fidelity mix from Supplier K. GC bias plots were generated, with %GC content of 100 bp windows on the X axis. Normalized coverage is indicated for the mixes and can be compared to a PCR-free reference library (representing unbiased coverage). The results illustrate SimpliFi HS Mix has a lower bias across the entire range of GC content, compared to the PCR-amplified library from supplier K, especially for GC percentages higher than 65%.PCR Enzyme Guide Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research PCR Selection Chart Select the best reagent for your research