产品说明
DescriptionPrecise, highly reproducible and fast genotyping of sequence variants through high resolution melting.Product HighlightsAccurate – clear discrimination of even the most challenging sequence differencesReproducible – unparalleled consistency between replicate difference plots for increased confidence in sample characterizationSensitive – efficient detection from even very limiting amounts of sampleFlexible – enables reliable characterization of a broad range of sequence differencesFast – rapid PCR amplification prior to high resolution melting, enabling higher throughputProduct DescriptionSensiFAST HRM Kit has been developed for detailed characterization of samples according to their base composition, length and GC content by high resolution melting. The latest advances in buffer chemistry and enhancers, together with an antibody-mediated hot-start DNA polymerase system, ensure the SensiFAST HRM Kit delivers reproducible, accurate HRM analysis.SensiFAST HRM Kit contains EvaGreen®, a third generation, saturating fluorescent dye which selectively binds to double-stranded DNA. In contrast to dyes such as SYBR® Green I, EvaGreen can be used at higher concentrations without inhibiting PCR and shows equal binding affinity for GC-rich and AT-rich regions. The combination of our SensiFAST DNA Polymerase, unique buffer system and EvaGreen dye enables amplification and discrimination of even the most challenging sequence differences, such as class IV SNPs without sequence preference. Since it does not require expensive labelled oligonucleotide probes, SensiFAST HRM Kit is also a cost effective alternative to traditional probe based genotyping methods.Request InfoApplicationsSNP genotypingCharacterization of haplotype blocksDNA mappingDetection of insertions, deletions and translocationsDNA fingerprintingDNA methylation analysisGene scanningSpecies identificationAllelic prevalence population studiesScreening for loss of heterozygosity Fig. 1 Highly successful genotyping of a Class 4 (A/T) SNP using SensiFAST HRMClass 4 SNPs are the most challenging to differentiate; having Tm changes typically less than 0.2oC. The three different genotypes of an A>T SNP were analysed on the Rotor-Gene 6000, using SensiFAST HRM Kit. A difference plot of this data illustrates the effect of subtracting an average heterozygous curve from all curves and plotting the results.Introduction to SensiFAST Overview, features and benefits of the SensiFAST product familyReal-Time PCR Selection ChartOne-step Vs. Two-step real-time RT PCR A discussion of the pros and cons of each detection strategy.
Bioline成立于1992年，总部设在英国伦敦；随着时间推移，信息化的高速发展，Bioline目前已经成国际化公司，其分部设在美国，德国，法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司，是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量，定制和OEM的dNTP，我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力，研发和制造的主要活动领域包括：实时定量PCR试剂盒，DNA聚合酶，逆转录酶，克隆试剂，核酸分离，超纯核苷酸合成，DNA与RNA分子量Marker，感受态细胞，分子生物学试剂，蛋白的表达及纯化。2010年，Meridian Life Science收购了 Bioline 公司。
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