DescriptionMyTaq Extract-PCR Kit provides quick and easy extraction and amplification of DNA from a variety of tissue types. The MyTaq Extract-PCR Kit maximizes sensitivity while simultaneously minimizing contamination risks to deliver greater experiment success rates.Product HighlightsFast – single-tube protocol that eliminates wash steps, giving high-yield, PCR-ready DNA in just 15 minutesSimple – few protocol steps greatly reduce the risk of sample loss and contamination and minimizes manual effortSensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targetsSpecific - MyTaq HS DNA Polymerase is an antibody-mediated hot-start enzyme that remains completely inactive during PCR set-up to prevent non-specific amplificationFlexible – ideal for amplifying any target up to 5 kb from DNA extracted from mammalian tissue samplesConvenient – mastermix facilitates PCR set-up and includes a red dye for improved pipetting ease / accuracy and to enable direct gel loadingProduct DescriptionMany DNA extraction methods can be laborious and time consuming or involve the use of hazardous chemicals. MyTaq™ Extract-PCR Kit offers a rapid, easy and safer alternative for the extraction and amplification of DNA from a variety of tissue types. MyTaq Extract-PCR Kit is particularly suited to solid tissues such as mouse tail or mouse ear. The DNA extractions are performed in a single-tube, without the need for multiple washing steps, greatly reducing the risk of sample loss and contamination.The extracted DNA is amplified in a proprietary buffer system using MyTaq HS Red Mix, the latest generation of very high-performance polymerase unique to Meridian. To further reduce non-specific amplification, MyTaq HS uses antibody hot-start technology. The advanced formulation of MyTaq HS Red Mix allows fast cycling conditions to be used, greatly reducing the reaction time without compromising PCR specificity or yield.The rapid MyTaq Extract-PCR Kit maximizes sensitivity while minimizing contamination risks to deliver improved success rates in applications such as mouse DNA characterization. The single tube lysis protocol and MyTaq HS Red Mix maximize sensitivity, while minimizing contamination risks and significantly reduce reaction times as well as delivering improved success rates in protocols such as mouse DNA characterizationRequest InfoApplicationsHigh-throughput genotyping from mammalian tissueDetection of transgenesKnockout analysis MyTaq Extract-PCR was used to extract and amplify genomic DNA from 3 mg pieces of mouse tail. A two-fold serial dilutions of the extract was prepared, then amplified (Lanes 1 – 11) used in PCR reactions containing MyTaq HS Mix and primers for amplification of a 1 kb fragment from the mouse γ-actin (Lanes 1 – 11). Marker is EasyLadder I (M). The results illustrate increased yield across all template input amounts versus all other kits tested. Comparison of amplification of fragments of the mouse cortexin-1 gene (CTXN1). The MyTaq Extract-PCR Kit and a corresponding kit from Supplier S were used to extract and amplify genomic DNA from 3 mg pieces of mouse tail according to the manufacturers’ instructions. Two-fold serial dilutions (Lanes 1 – 12) were used for the amplification of a 1 kb fragment (A) and a 2 kb fragment (B) from the mouse CTXN1 gene. The extraction protocol and PCR conditions used in each case were those recommended by each supplier. Marker - EasyLadder I (M). The results illustrate increased yield across all template input amounts versus the kit from Supplier S.Introduction to MyTaq Overview, features and benefits of the MyTaq product familyPCR Enzyme Guide Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research Application NoteMyTaq™ Extract-PCR Kit