产品说明
DescriptionA unique blend of high-performance hot-start MyTaq™ HS DNA Polymerase and a proprietary proofreading enzyme, specifically developed for long PCR applicationsProduct HighlightsEfficient – specifically developed to give reliable amplification of fragments between 10 kb and 25 kb in lengthSpecific – incorporates an antibody-mediated hot-start enzyme blend that remains completely inactive during PCR set-up to prevent non-specific amplificationFast – rapid enzyme activation and highly efficient amplification support reduced time to resultsFlexible – ideal for amplifying a broad range of large fragments from even complex targets including human genomic DNAAccurate – possesses 3’ – 5’ proofreading exonuclease activity for increased fidelity, thereby enabling cloning of long PCR productsProduct DescriptionRANGER DNA Polymerase is recommended for all long PCR applications. RANGER is an easy-to-use, high-performance enzyme blend, specifically developed to amplify fragments up to 25 kb in length. RANGER contains a unique combination of a highly-efficient DNA polymerases and novel buffer system that deliver the improved efficiency necessary for reliable amplification of longer amplicons.RANGER is an antibody-mediated hot-start enzyme blend that eliminates non-specific amplification during reaction set-up. The inactivated enzymes do not possess polymerase activity, thereby preventing the non-specific amplification, such as primer-dimer formation, that often hinder long PCR reactions from the start.RANGER is provided with a specially formulated reaction buffer containing dNTPs, MgCl2 and enhancers at optimal concentrations, which deliver increased processivity, sensitivity and specificity, thereby enabling successful amplification of long human genomic DNA fragments. This minimizes the requirements for PCR optimization, in turn reducing time to results and inconvenience of performing unnecessary repeats.Request InfoApplicationsLong PCRTA cloning A 10 kb fragment (A) and a 23 kb fragment (B) of human b-globin (HbG) gene, were amplified using RANGER DNA Polymerase and polymerases from Suppliers Q, S and I. A three-fold serial dilution of genomic DNA (5 ng - 6 pg and 0 pg) was used as template (lanes 1-8 respectively). HyperLadder 1 kb (M). The results demonstrate that RANGER can be used to amplify longer fragments, up to 23 kb from genomic DNA, compared to alternative Long PCR polymerases.PCR Enzyme Guide Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research PCR Selection Chart Select the best reagent for your research
Bioline成立于1992年，总部设在英国伦敦；随着时间推移，信息化的高速发展，Bioline目前已经成国际化公司，其分部设在美国，德国，法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司，是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量，定制和OEM的dNTP，我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力，研发和制造的主要活动领域包括：实时定量PCR试剂盒，DNA聚合酶，逆转录酶，克隆试剂，核酸分离，超纯核苷酸合成，DNA与RNA分子量Marker，感受态细胞，分子生物学试剂，蛋白的表达及纯化。2010年，Meridian Life Science收购了 Bioline 公司。
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