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bioline/Random Hexamer Primers/25µg/BIO-38028
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产品说明
DescriptionRandom Hexamer Primers are a mixture of oligonucleotides representing all possible sequence for that size. Random Primers can be used to prime synthesis in oligo-labeling similar to using hexamers and cDNA synthesis.Product HighlightsMixture of oligonucleotides -for RNA missing a polyA tail such as bacterial RNABinds randomly -for RNA with strong secondary structure Product DescriptionRandom Hexamer Primers consist of a mixture of oligonucleotides representing all possible hexamer sequences. Random Hexamer Primers are commonly used for priming single-stranded DNA or RNA for extension by DNA polymerases or reverse transcriptases.During cDNA generation, random priming gives random coverage to all regions of the RNA to generate a cDNA pool containing various lengths of cDNA. Random priming is incapable of distinguishing between mRNA and other RNA species present in the reaction.Primer Sequence: 5´ – d (NNNNNN) –3´ N = G, A, T or CA mixture of random hexamer primers and oligo(dT) may improve the sensitivity of cDNA synthesis.Request InfoApplicationsDNA synthesis using Klenow fragment with DNA templatesDNA probe synthesis for use in Northern and Southern blots, and in situ hybridization applicationsPartially degraded RNA samplesRNA without poly(A) tail such as ribosomal RNAsRNA with strong secondary structureTarget regions at 5´ end of a long messenger RNA transcript

Bioline成立于1992年,总部设在英国伦敦;随着时间推移,信息化的高速发展,Bioline目前已经成国际化公司,其分部设在美国,德国,法国和澳大利亚。BIOLINE是一家通过ISO9001:2008认证的公司,是世界上为数不多的超纯的dNTPs制造商之一。BIOLINE能够为大型实验室提供批量,定制和OEM的dNTP,我们已经开发和制造多种专利试剂和试剂盒。我们的核心竞争力,研发和制造的主要活动领域包括:实时定量PCR试剂盒,DNA聚合酶,逆转录酶,克隆试剂,核酸分离,超纯核苷酸合成,DNA与RNA分子量Marker,感受态细胞,分子生物学试剂,蛋白的表达及纯化。2010年,Meridian Life Science收购了 Bioline 公司。