DescriptionMyTaq™ Plant-PCR Kit offers fast, highly-specific, direct PCR from a wide range of plant leaf samples. The novel buffer system circumvents the need for additional PCR stabilizers and traditional purification steps, thereby avoiding a complex extraction process.Product HighlightsFast- eliminate complex, slow and costly DNA extraction steps, thereby reducing time to resultsSensitive – incorporates MyTaq HS DNA Polymerase that exhibits increased affinity for DNA, thereby improving yield of even the most challenging targetsRobust- specially developed to overcome common plant-derived PCR inhibitors such as polyphenolics and polysaccharides for highest PCR success rates and improved sensitivityVersatile- perfect for a wide range of plant species, avoiding the need for further optimization, thereby minimizing setup time and reducing costSimple- ideal for fast genotyping in plant genetic studies, mutation detection, confirming transgenic plant and knockout analysisProduct DescriptionMyTaq™ Plant PCR Kit is recommended for fast, specific and direct PCR from a wide range of plant leaf samples. MyTaq Plant-PCR Kit incorporates MyTaq HS DNA Polymerase, a next-generation hot-start polymerase that delivers highly-specific PCR amplification. Furthermore, MyTaq HS has an increased affinity for template DNA, giving a high PCR product yield for most challenging templates. MyTaq HS DNA Polymerase has been developed to give more robust amplification than other commonly-used polymerases meaning it performs reliably even in the presence of PCR inhibitors.MyTaq Plant-PCR Kit includes novel buffer system that replaces the need for complicated extraction or purification steps, including freezing of plant tissues with liquid nitrogen, mechanical disruption, organic extraction or column DNA purification. The advanced formulation of MyTaq Plant-PCR Kit also allows fast cycling conditions to be used, without compromising PCR specificity and yield.MyTaq Plant-PCR Kit has been developed to tolerate the PCR inhibitors typically present in plant samples, including polyphenolics and polysaccharides, thereby delivering significantly improved assay sensitivity and reproducibility. MyTaq Plant-PCR Kit increases amplification success rates from different plant types and its speed and high specificity makes it ideal for high-throughput genotyping assays.Meridian R&D has obtained successful results when using the MyTaq Plant-PCR Kit to conduct direct PCR from a very broad range of species.These include tomato, rice, sugarcane, maize, potato, soya, wheat, barley, cashew, oak, dwarf umbrella tree, Australian laurel, red-fruit saw-sedge, blueberry lily, she-oaks, coastal rosemary, burrawang, eucalyptus/gum tree, seaweed/crayweed, coastal banksia, bottle brush and honey gem.For more information, please inquire.As part of our test and review campaign, many of our customers have shared their positive experiences of the MyTaq Plant-PCR Kit.Our customers have also reported success for direct PCR from wheat, rosemary, cotton, tobacco, citrus, kangkong, mousear cress (Arabidopsis), cranberry, primrose, blueberry, American bellflower and several grass and fungus species.Request InfoApplicationsGenotyping for plant genetic studyMutation detectionTransgenic detectionKnockout analysis Fig. 1 Increasing concentration of inhibitors in a reaction Amplification of increasing numbers of rice leaf punches.Increasing the number of leaf punches also increases the concentration of PCR inhibitors in the reaction.The thermal cycling conditions were set according to the manufacturers recommendations. The results show that MyTaq Plant-PCR exhibits improved tolerance to inhibitors, without compromising the PCR efficiency. Fig. 2 Sensitivity of amplification from different plant samples Amplification of 0.5 kb fragments directly from leaf punches (1.2 mm diameter) from sugarcane, rice, tomato and maize. 50 μL PCR reactions were set up and run according to the manufacturers recommendations. A two-fold serial dilution of the PCR product was run on an agarose gel to illustrate the higher yield and therefore higher sensitivity of the MyTaq Plant-PCR over other suppliers.MyTaq™ Plant-PCR Kit Workflow Dr. Steve Hawkins delivers a simple protocol for Direct Plant PCR using MyTaq™ Plant-PCR Kit.Introduction to MyTaq Overview, features and benefits of the MyTaq product family Poster A Powerful Tool for Plant Genome Analysis. PCR Enzyme Guide Download the PCR Enzyme Guide with detailed product descriptions and performance data to help you choose the best product for your research PCR Selection Chart Select the best reagent for your research