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Catalog #22-ALSHU-E01SpeciesHumanRegulatory StatusResearch Use Only. Not for Use in Diagnostic Procedures.Product DistributionAvailable WorldwideRange0.53-200 ng/mLSensitivity0.53 ng/mLSizes96 WellsSample TypesCitrate Plasma, EDTA Plasma, Heparin Plasma, SerumIncubation Time3 hrSample Size10µLInstructions for UseClick here to viewSafety Data SheetClick here to view - Additional Information
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The Insulin-like Growth Factors (IGF) – I and II are bound to specific binding proteins in circulation(IGFBP). Until today seven different proteins have been identified IGFBP-1 to 7. IGF bioavailability, transport and storage is regulated or facilitated by these binding proteins which areexpressed differentially according physiological and developmental requirements. The most abundantIGFBP in circulation is IGFBP-3. Together with IGFBP-5 it is able to form the so called ternary complexwith IGF and the acid-labile subunit (ALS). In the circulation nearly all IGF is bound in this ternarycomplex and thus not able to cross the endothelial barrier. Only very small amounts of IGF or IGFBP-3exist outside this complex. The acid-labile subunit is an important part of the IGF-storagemechanism in circulation. In ALS deficiency or in ALS knock-out mice the concentration of IGF andIGFBP-3 in the circulation is significantly decreased resulting in impaired growth.The acid-labile Subunit, is a synthesized as propeptide of 605 amino acids. The signal peptide,necessary for ALS secretion (AA 1-27) cleaved off enduring the transport process (Swiss-Prot P35858Version 82). The mature protein consists of 578 amino acids and contains about 20 leucin richsequence repeats. Beside the leucin-rich repeats several potential N-linked glycosylation sides havebeen described. Miller BS et al. were able to demonstrate that incomplete glycolsylation of IGFs, ALSand IGFBP-3 results in a decreased serum concentration of these proteins. Oral mannose therapyresulted in a partial normalization of the glycosylation pattern and went along with improved growth.Mutations in or the complete knock out of the ALS gene result in IGF / IGFBP-3 deficiency andtherewith in distribution of growth. Beside growth also other endocrine axes may be involved. Inprimary ALS deficiency hyperinsulinemia could be observed. Further, the HGH-IGF-IGFBPsystemseems to be of relevance in coronary disease.The first ALS immunoassay was described by Baxter RC in 1990 [6]. By this in-houseradioimmunoassay it was shown that ALS is present in high concentrations in serum (50µg/mL) ofhealthy humans. But not detectable in other body fluids like amniotic fluid, cerebrospinal fluid or seminal plasma – in spite of the fact that these body fluids contain high level IGFBP-3.
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各位师兄师姐:
我近期负责的课题,需要进行人类血液淋巴细胞和DC细胞的分离和培养,但我在方面懂的不多,想求助这方面的大神们。后续我需要做RT-qPCR,western-blot、免疫荧光等实验,我应该去多少ml全血比较好?全血怎么保存?怎么进行分离?后续怎么培养和保存?
谢谢!
我的步骤:
1、16-22天小鼠颈椎脱臼法处死后,75%酒精浸泡30秒消毒,睾丸取出后置预冷PBS,40分钟(路上所需时间)后开始处理。
2、剔除白膜,剪碎睾丸。0.25%胰酶(含edta)1.5ml/个睾丸37°C消化30-50分钟,等体积DMEM/F12含10%FBS终止消化,1000r/MIN离心5分钟,弃上液。
3、加0.1%胶原酶消化30分钟,过200目网筛,1000r/MIN离心5分钟,弃上液。
4、DMEM/F12含10%FBS5ml重悬后转如培养瓶,入孵育箱37°C5%CO2培养。
请教各位,我到底是哪里有问题,是胰酶消化的时间长了吗?
请大神前辈指点指点,已经研三了,实验很不顺利,再这样都要延期的节奏了。请问最近急性分离出来的大鼠心肌细胞很不耐钙,做膜片钳封接不上可能是什么原因造成的?谢谢了!
前辈们好,近期老板布置任务需要分离小鼠肝脏细胞,查了好多文献,都没有写胶原酶的cat#,只写sigma订购,想请教下,是否有人做过,可以提供下货号和使用方法吗?十分感谢。另外,文中还提到digitonin,如果有一起用到的话麻烦帮我查看下,谢谢。
大概步骤如下:c57小鼠大概六七只,酒精浸泡后不处死取出,迅速打开胸腔取出心脏放入冰PBS冲洗。待心脏取出完毕,冲洗移入有PBS青霉素小瓶中用眼科剪剪成较小组织块。
之后加入0.1%胰酶消化(之前浓度为0.25%,活细胞数量更少。)吹打1min.放入孵箱中3min.弃去上清,加入∥型胶原酶(0.1%)吹打10Min,放入孵箱15Min。取出后加入1ml胰酶。吹打1Min后加入含血清F12培养液终止消化,800转离心5Min。之后取沉淀重悬种板。90Min差速贴壁法纯化心肌细胞。
48h后只有少量(个位数)细胞贴壁,呈三角形。
第一次用0.25%胰酶时离心后有大量鼻涕样物质。
第二次胰酶浓度减小后没有,但细胞沉淀少,且为半透明微白。是不是离心时间不够。
感觉细胞整体状态都不好,几次都没有大量心肌细胞,更不用说观察到心肌细胞搏动。
老师们能不能帮忙分析一下是哪里的原因呢。
有没有比较稳定的小鼠乳鼠心肌细胞分离步骤呢。
希望大神快点出现。
想用二步酶消法来分离毛乳头细胞,一个毛囊大概有多少个毛乳头细胞?处于生长期的毛囊在显微镜下有什么特点?先谢谢大神们指点
经过无数次的大鼠肝脏插针灌注,忍受过实验结果不理想的打击和折磨,今天终于成功了,特分享些相关图片以鼓励还在奋战中的网友们。
近期根据经验总结了原代肿瘤细胞分离培养的2个关键因素。
1、组织的活性:如果我们取的都是坏死的组织或者结缔组织,我相信任何方法都不可能分离出来活的细胞的。另外,取完的组织要冷藏存放,并且越早分离越好,最好在24h内,最久不要超过48h。
2、组织的消化:在组织消化前,组织剪的越碎越好(1mm3左右为宜),组织的大小决定了组织的消化时间,不同组织消化时间差别较大:例如肺癌在2h-2.5h;食管癌在1个多小时。
希望可以帮到各位研友,另外如果大家有更好的经验可以一起来讨论!
最近在做黄体化颗粒细胞分离培养,但是注射器吸取大卵泡液或者从下颗粒细胞离心时就会凝固成果冻状,大家有碰到过这样的事情么?已经好几次了,一直是这样,大家给支个招吧
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