CBHAHDAC inhibitor |
Sample solution is provided at 25 µL, 10mM.
Quality Control & MSDS
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- Purity = 98.00%
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- MSDS (Material Safety Data Sheet)
Chemical structure
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Cas No. | 174664-65-4 | SDF | Download SDF |
Synonyms | Histone Deacetylase Inhibitor II,m-Carboxycinnamic Acid bis-Hydroxamide | ||
Chemical Name | N-hydroxy-3-[3-(hydroxyamino)-3-oxo-1-propen-1-yl]-benzamide | ||
Canonical SMILES | ONC(=O)C=C/c1cccc(c1)C(=O)NO | ||
Formula | C10H10N2O4 | M.Wt | 222.2 |
Solubility | ≤20mg/ml in DMSO;20mg/ml in dimethyl formamide | Storage | Store at -20°C |
Physical Appearance | A crystalline solid | Shipping Condition | Evaluation sample solution : ship with blue ice.All other available size:ship with RT , or blue ice upon request |
General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while.Stock solution can be stored below -20℃ for several months. |
CBHA is a potent and selective inhibitor with ID50 values of 0.01 and 0.07 μM for HDAC1 and HDAC3, respectively [1].
Histone deacetylases (HDACs) are a class of enzymes that remove acetyl groups from lysine amino acid on histone, allowing the histones to wrap the DNA more tightly. HDAC inhibitors hyperacetylate histones and increase transcriptional activity in target genes. HDAC inhibitors have both apoptotic and differentiating effects on various tumor cells [2].
M-carboxycinnamic acid bishydroxamide (CBHA) is a potent and selective HDAC inhibitor. CBHA is a hybrid polar compound that is synthesized to induce terminal differentiation and/or apoptosis in various transformed cells. In MEL cells, CBHA caused accumulation of acetylated histone H4 [1]. In neuroblastoma cell lines, CBHA induced acetylated histone H3 and H4 accumulation. CBHA also led to extensive cell death with LD50 ranged between 1 μM and 4 μM, and induced apoptosis [2].
In human neuroblastoma xenograft SCID mice, CBHA (100 mg/kg and 200 mg/kg) resulted in reductions of average final tumor volume of ~50% and 75%, respectively [3].
References:[1]. Richon VM1, Emiliani S, Verdin E, et al. A class of hybrid polar inducers of transformed cell differentiation inhibits histone deacetylases. Proc Natl Acad Sci U S A. 1998 Mar 17;95(6):3003-7.[2]. Glick RD1, Swendeman SL, Coffey DC, et al. Hybrid polar histone deacetylase inhibitor induces apoptosis and CD95/CD95 ligand expression in human neuroblastoma. Cancer Res. 1999 Sep 1;59(17):4392-9.[3]. Coffey DC, Kutko MC, Glick RD, et al. The histone deacetylase inhibitor, CBHA, inhibits growth of human neuroblastoma xenografts in vivo, alone and synergistically with all-trans retinoic acid. Cancer Res. 2001 May 1;61(9):3591-4.
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各位师兄师姐:
我近期负责的课题,需要进行人类血液淋巴细胞和DC细胞的分离和培养,但我在方面懂的不多,想求助这方面的大神们。后续我需要做RT-qPCR,western-blot、免疫荧光等实验,我应该去多少ml全血比较好?全血怎么保存?怎么进行分离?后续怎么培养和保存?
谢谢!
我的步骤:
1、16-22天小鼠颈椎脱臼法处死后,75%酒精浸泡30秒消毒,睾丸取出后置预冷PBS,40分钟(路上所需时间)后开始处理。
2、剔除白膜,剪碎睾丸。0.25%胰酶(含edta)1.5ml/个睾丸37°C消化30-50分钟,等体积DMEM/F12含10%FBS终止消化,1000r/MIN离心5分钟,弃上液。
3、加0.1%胶原酶消化30分钟,过200目网筛,1000r/MIN离心5分钟,弃上液。
4、DMEM/F12含10%FBS5ml重悬后转如培养瓶,入孵育箱37°C5%CO2培养。
请教各位,我到底是哪里有问题,是胰酶消化的时间长了吗?
请大神前辈指点指点,已经研三了,实验很不顺利,再这样都要延期的节奏了。请问最近急性分离出来的大鼠心肌细胞很不耐钙,做膜片钳封接不上可能是什么原因造成的?谢谢了!
前辈们好,近期老板布置任务需要分离小鼠肝脏细胞,查了好多文献,都没有写胶原酶的cat#,只写sigma订购,想请教下,是否有人做过,可以提供下货号和使用方法吗?十分感谢。另外,文中还提到digitonin,如果有一起用到的话麻烦帮我查看下,谢谢。
大概步骤如下:c57小鼠大概六七只,酒精浸泡后不处死取出,迅速打开胸腔取出心脏放入冰PBS冲洗。待心脏取出完毕,冲洗移入有PBS青霉素小瓶中用眼科剪剪成较小组织块。
之后加入0.1%胰酶消化(之前浓度为0.25%,活细胞数量更少。)吹打1min.放入孵箱中3min.弃去上清,加入∥型胶原酶(0.1%)吹打10Min,放入孵箱15Min。取出后加入1ml胰酶。吹打1Min后加入含血清F12培养液终止消化,800转离心5Min。之后取沉淀重悬种板。90Min差速贴壁法纯化心肌细胞。
48h后只有少量(个位数)细胞贴壁,呈三角形。
第一次用0.25%胰酶时离心后有大量鼻涕样物质。
第二次胰酶浓度减小后没有,但细胞沉淀少,且为半透明微白。是不是离心时间不够。
感觉细胞整体状态都不好,几次都没有大量心肌细胞,更不用说观察到心肌细胞搏动。
老师们能不能帮忙分析一下是哪里的原因呢。
有没有比较稳定的小鼠乳鼠心肌细胞分离步骤呢。
希望大神快点出现。
想用二步酶消法来分离毛乳头细胞,一个毛囊大概有多少个毛乳头细胞?处于生长期的毛囊在显微镜下有什么特点?先谢谢大神们指点
经过无数次的大鼠肝脏插针灌注,忍受过实验结果不理想的打击和折磨,今天终于成功了,特分享些相关图片以鼓励还在奋战中的网友们。
近期根据经验总结了原代肿瘤细胞分离培养的2个关键因素。
1、组织的活性:如果我们取的都是坏死的组织或者结缔组织,我相信任何方法都不可能分离出来活的细胞的。另外,取完的组织要冷藏存放,并且越早分离越好,最好在24h内,最久不要超过48h。
2、组织的消化:在组织消化前,组织剪的越碎越好(1mm3左右为宜),组织的大小决定了组织的消化时间,不同组织消化时间差别较大:例如肺癌在2h-2.5h;食管癌在1个多小时。
希望可以帮到各位研友,另外如果大家有更好的经验可以一起来讨论!
最近在做黄体化颗粒细胞分离培养,但是注射器吸取大卵泡液或者从下颗粒细胞离心时就会凝固成果冻状,大家有碰到过这样的事情么?已经好几次了,一直是这样,大家给支个招吧
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