This product requires shipping in dry ice and an additional shipping charge will be made.
Product Type:
Microsome
Description:
Please note that the Silensomes™ CONTROL (Product Code: SIL000) will also need to be purchased with this product
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Supplier:
Biopredic
Product Category:
Cell Fractions & Lysates > Human Cell Fractions
Silensomes are human pooled liver microsomes in which one specific CYP has been chemically knocked out. Silensomes is a single unique and representative model, capable of directly quantifying and predicting the contribution of CYP enzymes in drug metabolism
Further information about Silensomes
Silensomes Reference
Silensomes User Guide
Product Code:
SIL270
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Contributor: Suprya JayadevDate: November 4, 19911) Grow cells to a density of 5-8 X 105 cells/ml in RPMI 1640 containing serum.2) Pellet cells and wash 1 time 查看更多>
Protocol Wash cells with PBS and trypsinize to a single cell suspension. Count an aliquot on a hemocytometer. Meanwhile centrifuge the cells (e.g. 1000 RPM, 5 查看更多>
Fixation1) Collect 2 X 106 cells.2) Pellet cells by spinning at 1,000 rpm, 4°C for 5 minutes.3) Resuspend cell pellet in 1 ml of cold PBS.4) Fix cells by addin 查看更多>
Preparation of cell lysates from E. coli by sonicationMaterialsLysis buffer50 mM Tris-HCl pH 7.5 50-200 mM NaCl*5 mM DTT1 mM PMSF*The NaCl concentration used i 查看更多>